Dose enhancement effect of anticaner drugs associated with increased temperature in vitro
Aim: To evaluate in vitro the influence of elevated temperature (42 °C for 60 min) on the action of anticancer drugs doxorubicin, vinorelbine, carboplatin, ifosfamide, etoposide, oxaliplatin, docetaxel and gemcitabine. Methods: HeLa tumor cell cultures, 24 h after seeding, were incubated for 60 min...
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| Дата: | 2008 |
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| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2008
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139227 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Dose enhancement effect of anticaner drugs associated with increased temperature in vitro / Yu.Р. Istomin, E.A. Zhavrid, E.N. Alexandrova, O.P. Sergeyeva, S.V. Petrovich // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 56–59. — Бібліогр.: 28 назв. — англ. |
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irk-123456789-1392272018-06-21T03:05:46Z Dose enhancement effect of anticaner drugs associated with increased temperature in vitro Istomin, Yu.P. Zhavrid, E.A. Alexandrova, E.N. Sergeyeva, O.P. Petrovich, S.V. Uncategorized Aim: To evaluate in vitro the influence of elevated temperature (42 °C for 60 min) on the action of anticancer drugs doxorubicin, vinorelbine, carboplatin, ifosfamide, etoposide, oxaliplatin, docetaxel and gemcitabine. Methods: HeLa tumor cell cultures, 24 h after seeding, were incubated for 60 min with different concentrations of chemotherapeutical drugs at a temperature of 37 °C or 42 °C. 48 h later the number of viable cells in the flasks were counted using trypan-blue exclusion on a hemacytometer. Results: Hyperthermia alone caused only 10–20% growth inhibition of cell culture. All the chemotherapeutic drugs used demonstrated a dose enhancement effect at elevated temperature. Thermal enhancement ratio for cell proliferation for oxaliplatin, vinorelbine, carboplatin and ifosfamide exceeded 4, for doxorubicin and gemcitabine exceeded 2. Thermal enhancement ratio for cell death did not exceed 1.4. Conclusion: Synergism of hyperthermia and chemotherapeutical drugs was clearly demonstrated for oxaliplatin, vinorelbine, carboplatin, ifosfamide and to a lesser extent for doxorubicin and gemcitabine. Enhancement of the cytostatic effect of anticancer drugs by hyperthermia was more prominent than their cytotoxic effect. Цель: изучить in vitro влияние повышенной температуры (42 °C в течение 60 мин) на действие противоопухолевых препаратов: доксорубицина, винорельбина, карбоплатина, ифосфамида, этопозида, оксалиплатина, доцетаксела и гемцитабина. Методы: культуру опухолевых клеток HeLa через 24 ч после рассева инкубировали в течение 60 мин с различными концентрациями химиотерапевтических препаратов при температуре 37 °C или 42 °C. Спустя 48 ч подсчитывали количество живых клеток во флаконах, используя гемоцитометр и метод исключения красителя трипанового синего. Результаты: гипертермия сама по себе вызывала 10–20% угнетение роста культуры клеток. У всех исследованных химиотерапевтических препаратов отмечали эффект усиления при повышенной температуре. Коэффициент теплового усиления в отношении клеточной пролиферации для оксалиплатина, винорельбина, карбоплатина и ифосфамида превысил 4,0, для доксорубицина и гемцитабина — 2,0. Коэффициент теплового усиления в отношении гибели клеток не превышал 1,4. Выводы: синергизм гипертермии и химиотерапевтических препаратов продемонстрирован для оксалиплатина, винорельбина, карбоплатина, ифосфамида, в меньшей степени — для доксорубицина и гемцитабина. Усиление цитостатического эффекта противоопухолевых препаратов под действием гипертермии было более выраженным, чем их цитотоксического эффекта. 2008 Article Dose enhancement effect of anticaner drugs associated with increased temperature in vitro / Yu.Р. Istomin, E.A. Zhavrid, E.N. Alexandrova, O.P. Sergeyeva, S.V. Petrovich // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 56–59. — Бібліогр.: 28 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139227 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Uncategorized Uncategorized |
| spellingShingle |
Uncategorized Uncategorized Istomin, Yu.P. Zhavrid, E.A. Alexandrova, E.N. Sergeyeva, O.P. Petrovich, S.V. Dose enhancement effect of anticaner drugs associated with increased temperature in vitro Experimental Oncology |
| description |
Aim: To evaluate in vitro the influence of elevated temperature (42 °C for 60 min) on the action of anticancer drugs doxorubicin, vinorelbine, carboplatin, ifosfamide, etoposide, oxaliplatin, docetaxel and gemcitabine. Methods: HeLa tumor cell cultures, 24 h after seeding, were incubated for 60 min with different concentrations of chemotherapeutical drugs at a temperature of 37 °C or 42 °C. 48 h later the number of viable cells in the flasks were counted using trypan-blue exclusion on a hemacytometer. Results: Hyperthermia alone caused only 10–20% growth inhibition of cell culture. All the chemotherapeutic drugs used demonstrated a dose enhancement effect at elevated temperature. Thermal enhancement ratio for cell proliferation for oxaliplatin, vinorelbine, carboplatin and ifosfamide exceeded 4, for doxorubicin and gemcitabine exceeded 2. Thermal enhancement ratio for cell death did not exceed 1.4. Conclusion: Synergism of hyperthermia and chemotherapeutical drugs was clearly demonstrated for oxaliplatin, vinorelbine, carboplatin, ifosfamide and to a lesser extent for doxorubicin and gemcitabine. Enhancement of the cytostatic effect of anticancer drugs by hyperthermia was more prominent than their cytotoxic effect. |
| format |
Article |
| author |
Istomin, Yu.P. Zhavrid, E.A. Alexandrova, E.N. Sergeyeva, O.P. Petrovich, S.V. |
| author_facet |
Istomin, Yu.P. Zhavrid, E.A. Alexandrova, E.N. Sergeyeva, O.P. Petrovich, S.V. |
| author_sort |
Istomin, Yu.P. |
| title |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| title_short |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| title_full |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| title_fullStr |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| title_full_unstemmed |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| title_sort |
dose enhancement effect of anticaner drugs associated with increased temperature in vitro |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2008 |
| topic_facet |
Uncategorized |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139227 |
| citation_txt |
Dose enhancement effect of anticaner drugs associated with increased temperature in vitro / Yu.Р. Istomin, E.A. Zhavrid, E.N. Alexandrova, O.P. Sergeyeva, S.V. Petrovich // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 56–59. — Бібліогр.: 28 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-10-18T21:19:39Z |
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2023-10-18T21:19:39Z |
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