VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro
Aim: To inhibit the gene expression of vascular endothelial growth factor (VEGF) with the RNA interference (RNAi) technique and explore its influence on the apoptosis, proliferation, vasculogenic mimicry of osteosarcoma in vitro. Methods: PSilencer-VEGF plasmid transfected into osteosarcoma cell lin...
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| Дата: | 2008 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2008
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| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139228 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro / J. Mei, Y. Gao, L. Zhang, X. Cai, Z. Qian, H. Huang, W. Huang // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 29–34. — Бібліогр.: 17 назв. — англ. |
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irk-123456789-1392282018-06-20T03:11:16Z VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro Mei, J. Gao, Y. Zhang, L. Cai, X. Qian, Z. Huang, H. Huang, W. Uncategorized Aim: To inhibit the gene expression of vascular endothelial growth factor (VEGF) with the RNA interference (RNAi) technique and explore its influence on the apoptosis, proliferation, vasculogenic mimicry of osteosarcoma in vitro. Methods: PSilencer-VEGF plasmid transfected into osteosarcoma cell line of MG63 was recombinated by gene cloning, and VEGF expression was examined by Western blotting after transfection. The biological features of transfected MG63 including proliferation, apoptosis and vasculogenic mimicry, were assessed by MTT assay, flowcytometer, annexin V-FITC/PI, light microscopy and HE staining. Results: The sequence of pSilencer-VEGF plasmid was confirmed by DNA sequencing. Sequence-specific siRNA targeting VEGF significantly decreases its expression in MG63 cells. Cell growth was inhibited and early apoptosis was induced compared with non-siRNA tansfected cells. Decreased VEGF expression was associated with reduced vasculogenic mimicry of MG63 cells. Conclusions: VEGF gene might be closely related to the apoptosis and proliferation of osteosarcoma cells, and appears to be crucial for formation of vasculogenic mimicry in osteosarcoma. Inhibition of VEGF expression by sequence-specific siRNA may become a novel approach for the treatment of VEGF-enriched neoplasma such as osteosarcoma. Цель: ингибировать експрессию фактора роста эндотелия сосудов (VEGF) с помощью метода РНК интерференции (RNAi) и изучить влияние введения сайленсинга siRNA-VEGF на апоптоз, пролиферацию, васкулярную мимикрию клеток остеосаркомы in vitro. Методы: плазмида PSilencer-VEGF, рекомбинированная за счет клонирования специфической siRNA последовательности, была трансфецирована в клеточную линию остеосаркомы MG63. Экспрессию VEGF после трансфекции проверяли с помощью Вестерн-блот анализа. Биологические особенности трансфецированных клеток MG63, а именно пролиферацию, апоптоз и васкулярную мимикрию, изучали с помощью MTТ-анализа, проточной цитофлуориметрии, аннексина V-FITC/пропидиум йодида, световой микроскопии и окрашивания гематоксилином и эозином (HE). Результаты: последовательность, клонированная в плазмиду pSilencer-VEGF, проверена при секвенировании ДНК. Специфические к VEGF последовательности siRNA значительно снижали его экспрессию клетками MG63. В сравнении с клетками, трансфeцированными плазмидой без siRNA, рост клеток с PSilencer-VEGF был ингибирован, а также в них был индуцирован ранний апоптоз. Ингибирование экспрессии VEGF было ассоциировано со снижением васкулярной мимикрии клеток MG63. Выводы: ген VEGF может быть тесно связан с регуляцией апоптоза и пролиферации клеток остеосаркомы и может быть ключевым регулятором васкулярной мимикрии. Ингибирование экспрессии VEGF с помощью специфических siRNA можно рассматривать как новый подход к лечению опухолей, характеризующихся повышенной экспрессией VEGF, таких как остеосаркома. 2008 Article VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro / J. Mei, Y. Gao, L. Zhang, X. Cai, Z. Qian, H. Huang, W. Huang // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 29–34. — Бібліогр.: 17 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139228 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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DSpace DC |
| language |
English |
| topic |
Uncategorized Uncategorized |
| spellingShingle |
Uncategorized Uncategorized Mei, J. Gao, Y. Zhang, L. Cai, X. Qian, Z. Huang, H. Huang, W. VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro Experimental Oncology |
| description |
Aim: To inhibit the gene expression of vascular endothelial growth factor (VEGF) with the RNA interference (RNAi) technique and explore its influence on the apoptosis, proliferation, vasculogenic mimicry of osteosarcoma in vitro. Methods: PSilencer-VEGF plasmid transfected into osteosarcoma cell line of MG63 was recombinated by gene cloning, and VEGF expression was examined by Western blotting after transfection. The biological features of transfected MG63 including proliferation, apoptosis and vasculogenic mimicry, were assessed by MTT assay, flowcytometer, annexin V-FITC/PI, light microscopy and HE staining. Results: The sequence of pSilencer-VEGF plasmid was confirmed by DNA sequencing. Sequence-specific siRNA targeting VEGF significantly decreases its expression in MG63 cells. Cell growth was inhibited and early apoptosis was induced compared with non-siRNA tansfected cells. Decreased VEGF expression was associated with reduced vasculogenic mimicry of MG63 cells. Conclusions: VEGF gene might be closely related to the apoptosis and proliferation of osteosarcoma cells, and appears to be crucial for formation of vasculogenic mimicry in osteosarcoma. Inhibition of VEGF expression by sequence-specific siRNA may become a novel approach for the treatment of VEGF-enriched neoplasma such as osteosarcoma. |
| format |
Article |
| author |
Mei, J. Gao, Y. Zhang, L. Cai, X. Qian, Z. Huang, H. Huang, W. |
| author_facet |
Mei, J. Gao, Y. Zhang, L. Cai, X. Qian, Z. Huang, H. Huang, W. |
| author_sort |
Mei, J. |
| title |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| title_short |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| title_full |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| title_fullStr |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| title_full_unstemmed |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| title_sort |
vegf-sirna silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2008 |
| topic_facet |
Uncategorized |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139228 |
| citation_txt |
VEGF-siRNA silencing induces apoptosis, inhibits proliferation and suppresses vasculogenic mimicry in osteosarcoma in vitro / J. Mei, Y. Gao, L. Zhang, X. Cai, Z. Qian, H. Huang, W. Huang // Experimental Oncology. — 2008. — Т. 30, № 1. — С. 29–34. — Бібліогр.: 17 назв. — англ. |
| series |
Experimental Oncology |
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2023-10-18T21:19:53Z |
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