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Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines

Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines

The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid ca...

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Main Authors: Zubenko, O.S., Semeniuk, D.O., Starenka, I.O., Pogribnyy, P.V.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2018
Series:Experimental Oncology
Subjects:
Short communications
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/139241
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spelling irk-123456789-1392412018-06-20T03:06:46Z Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines Zubenko, O.S. Semeniuk, D.O. Starenka, I.O. Pogribnyy, P.V. Short communications The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid carcinoma A431 cells and human breast adenocarcinoma MCF7 cells treated with cisplatin, methotrexate, doxorubicin or vincristine at the IC20 concentrations. Results: The cytostatic agents with different mechanisms of action affected differently expression of hBDs, dependent on the cell line. Mostly, cytostatic agents suppressed significantly expression of hBDs. In contrast, vincristine caused significant up-regulation of hBD-1 (12 fold, p < 0.05) and hBD-4 (2 fold, p < 0.05) in MCF7, and doxorubicin significantly enhanced expression of hBD-3 (2 fold, p < 0.05) and hBD-4 (> 10 fold, p < 0.05) in A431 cells. Conclusion: The results of this pilot study show that expression levels of hBD-1-4 may be altered upon treatment with cytostatic agents depending on nature of cells. 2018 Article Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines / O.S. Zubenko, D.O. Semeniuk, I.O. Starenka, P.V. Pogribnyy // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 79-81. — Бібліогр.: 6 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139241 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Short communications
Short communications
spellingShingle Short communications
Short communications
Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
Experimental Oncology
description The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid carcinoma A431 cells and human breast adenocarcinoma MCF7 cells treated with cisplatin, methotrexate, doxorubicin or vincristine at the IC20 concentrations. Results: The cytostatic agents with different mechanisms of action affected differently expression of hBDs, dependent on the cell line. Mostly, cytostatic agents suppressed significantly expression of hBDs. In contrast, vincristine caused significant up-regulation of hBD-1 (12 fold, p < 0.05) and hBD-4 (2 fold, p < 0.05) in MCF7, and doxorubicin significantly enhanced expression of hBD-3 (2 fold, p < 0.05) and hBD-4 (> 10 fold, p < 0.05) in A431 cells. Conclusion: The results of this pilot study show that expression levels of hBD-1-4 may be altered upon treatment with cytostatic agents depending on nature of cells.
format Article
author Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
author_facet Zubenko, O.S.
Semeniuk, D.O.
Starenka, I.O.
Pogribnyy, P.V.
author_sort Zubenko, O.S.
title Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_short Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_full Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_fullStr Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_full_unstemmed Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines
title_sort effect of cytostatic agents on expression levels of human beta-defensins-1-4 in a431 and mcf-7 cell lines
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2018
topic_facet Short communications
url http://dspace.nbuv.gov.ua/handle/123456789/139241
citation_txt Effect of cytostatic agents on expression levels of human beta-defensins-1-4 in A431 and MCF-7 cell lines / O.S. Zubenko, D.O. Semeniuk, I.O. Starenka, P.V. Pogribnyy // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 79-81. — Бібліогр.: 6 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:19:22Z
last_indexed 2023-10-18T21:19:22Z
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