Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients
Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the in...
Збережено в:
| Дата: | 2018 |
|---|---|
| Автори: | , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
|
| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139243 |
| Теги: |
Додати тег
Немає тегів, Будьте першим, хто поставить тег для цього запису!
|
| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| id |
irk-123456789-139243 |
|---|---|
| record_format |
dspace |
| spelling |
irk-123456789-1392432018-06-20T03:06:17Z Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients Buchynska, L.G. Brieieva, O.V. Original contributions Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the individual sensitivity to the 4OHE2 may determine the predisposition to endometrial cancer (EС). Aim: To analyze the sensitivity of peripheral blood lymphocytes (PBLs) of EC patients to the 4OHE2 and to evaluate the repair efficiency of 4OHE2-induced DNA damage. Materials and Methods: The study was performed on the PBLs of 53 EC patients and 20 healthy women. The level of DNA damage was measured using the comet assay and was expressed as % tail DNA. The DNA repair efficiency (%) was evaluated by determining the ratio between the amount of repaired DNA damage and the level of 4OHE2-induced damage that appeared after incubation of PBLs with 4OHE2. Results: In PBLs of EC patients, a higher level of 4OHE2-induced DNA damage (32.0 ± 2.2% tail DNA) and lower DNA repair efficiency (34.0 ± 4.5%) was observed compared to PBLs of healthy women (22.3 ± 2.3% tail DNA and 48.8 ± 4.5%, respectively). PBLs of EC patients with deep tumor invasion of myometrium were characterized by more prominent decrease of DNA repair than those with less invasive tumor (< ½ of myometrium) (20.9 ± 7.8 and 43.7 ± 6.7%, respectively). Furthermore, lower DNA repair efficiency was detected in the PBLs of EC patients with a family history of cancer compared to this parameter in patients with sporadic tumors (20.9±7.8 and 47.1 ± 5.5%, respectively). Conclusion: The PBLs of EC patients are characterized by increased sensitivity to the genotoxic effect of 4OHE2 and reduced repair efficiency regarding 4OHE2-induced DNA damage. A lower level of DNA repair is observed in EC patients with deep tumor myometrial invasion and a family history of cancer. 2018 Article Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139243 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Buchynska, L.G. Brieieva, O.V. Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients Experimental Oncology |
| description |
Background: The development of hormone-dependent cancers, including endometrial carcinomas, in great part may be mediated by the genotoxic effects of estrogen metabolites, among which 4-hydroxyestradiol (4OHE2) is characterized by the most prominent DNA-damaging properties. It is assumed that the individual sensitivity to the 4OHE2 may determine the predisposition to endometrial cancer (EС). Aim: To analyze the sensitivity of peripheral blood lymphocytes (PBLs) of EC patients to the 4OHE2 and to evaluate the repair efficiency of 4OHE2-induced DNA damage. Materials and Methods: The study was performed on the PBLs of 53 EC patients and 20 healthy women. The level of DNA damage was measured using the comet assay and was expressed as % tail DNA. The DNA repair efficiency (%) was evaluated by determining the ratio between the amount of repaired DNA damage and the level of 4OHE2-induced damage that appeared after incubation of PBLs with 4OHE2. Results: In PBLs of EC patients, a higher level of 4OHE2-induced DNA damage (32.0 ± 2.2% tail DNA) and lower DNA repair efficiency (34.0 ± 4.5%) was observed compared to PBLs of healthy women (22.3 ± 2.3% tail DNA and 48.8 ± 4.5%, respectively). PBLs of EC patients with deep tumor invasion of myometrium were characterized by more prominent decrease of DNA repair than those with less invasive tumor (< ½ of myometrium) (20.9 ± 7.8 and 43.7 ± 6.7%, respectively). Furthermore, lower DNA repair efficiency was detected in the PBLs of EC patients with a family history of cancer compared to this parameter in patients with sporadic tumors (20.9±7.8 and 47.1 ± 5.5%, respectively). Conclusion: The PBLs of EC patients are characterized by increased sensitivity to the genotoxic effect of 4OHE2 and reduced repair efficiency regarding 4OHE2-induced DNA damage. A lower level of DNA repair is observed in EC patients with deep tumor myometrial invasion and a family history of cancer. |
| format |
Article |
| author |
Buchynska, L.G. Brieieva, O.V. |
| author_facet |
Buchynska, L.G. Brieieva, O.V. |
| author_sort |
Buchynska, L.G. |
| title |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_short |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_full |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_fullStr |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_full_unstemmed |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| title_sort |
sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2018 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139243 |
| citation_txt |
Sensitivity to 4-hydroxyestradiol and dna repair efficiency in peripheral blood lymphocytes of endometrial cancer patients / L.G. Buchynska, O.V. Brieieva // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 68–72. — Бібліогр.: 28 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
AT buchynskalg sensitivityto4hydroxyestradiolanddnarepairefficiencyinperipheralbloodlymphocytesofendometrialcancerpatients AT brieievaov sensitivityto4hydroxyestradiolanddnarepairefficiencyinperipheralbloodlymphocytesofendometrialcancerpatients |
| first_indexed |
2023-10-18T21:19:23Z |
| last_indexed |
2023-10-18T21:19:23Z |
| _version_ |
1796152506544291840 |