Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach
Background: Among malignancies, lung cancer is a leading cause of death. Platinum-based therapeutic compounds used to treat lung cancer have not been able to increase the survival of patients and such compounds have a high incidence of adverse and toxic effects. It has been proposed that flavonoids...
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| Дата: | 2018 |
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| Автори: | , , , , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139247 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach / O. Varela-Castillo, P. Cordero, G. Gutiérrez-Iglesias, I. Palma, I. Rubio-Gayosso, E. Meaney, I. Ramirez-Sanchez, F. Villarreal, G. Ceballos, N. Nájera // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 19-23. — Бібліогр.: 25 назв. — англ. |
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irk-123456789-1392472018-06-20T03:06:26Z Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach Varela-Castillo, O. Cordero, P. Gutiérrez-Iglesias, G. Palma, I. Rubio-Gayosso, I. Meaney, E. Ramirez-Sanchez, I. Villarreal, F. Ceballos, G. Nájera, N. Original contributions Background: Among malignancies, lung cancer is a leading cause of death. Platinum-based therapeutic compounds used to treat lung cancer have not been able to increase the survival of patients and such compounds have a high incidence of adverse and toxic effects. It has been proposed that flavonoids such as catechins may significantly reduce the risk of developing cancer, alongside with other health benefits. The aim of this work was to determine the effect of (-)-epicatechin, the main flavanol found in cocoa, on the proliferation of the lung non-small cell adenocarcinoma cancer cell line A549, and to determine its effects when added simultaneously with cisplatin. Materials and Methods: Concentration-response curves for cisplatin and epicatechin were obtained, inhibitory concentrations calculated and an isobolographic analysis was then performed. Results: We found that epicatechin has a concentration-dependent inhibitory effect on proliferation of tumor cells and the isobolographic analysis reveals that the effect of its combination with cisplatin is synergistic. It was also observed that epicatechin promotes cell death by apoptosis. Conclusions: Epicatechin might be considered for future studies to explore its possible use as coadjuvant in cisplatin-based treatments. 2018 Article Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach / O. Varela-Castillo, P. Cordero, G. Gutiérrez-Iglesias, I. Palma, I. Rubio-Gayosso, E. Meaney, I. Ramirez-Sanchez, F. Villarreal, G. Ceballos, N. Nájera // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 19-23. — Бібліогр.: 25 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139247 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Varela-Castillo, O. Cordero, P. Gutiérrez-Iglesias, G. Palma, I. Rubio-Gayosso, I. Meaney, E. Ramirez-Sanchez, I. Villarreal, F. Ceballos, G. Nájera, N. Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach Experimental Oncology |
| description |
Background: Among malignancies, lung cancer is a leading cause of death. Platinum-based therapeutic compounds used to treat lung cancer have not been able to increase the survival of patients and such compounds have a high incidence of adverse and toxic effects. It has been proposed that flavonoids such as catechins may significantly reduce the risk of developing cancer, alongside with other health benefits. The aim of this work was to determine the effect of (-)-epicatechin, the main flavanol found in cocoa, on the proliferation of the lung non-small cell adenocarcinoma cancer cell line A549, and to determine its effects when added simultaneously with cisplatin. Materials and Methods: Concentration-response curves for cisplatin and epicatechin were obtained, inhibitory concentrations calculated and an isobolographic analysis was then performed. Results: We found that epicatechin has a concentration-dependent inhibitory effect on proliferation of tumor cells and the isobolographic analysis reveals that the effect of its combination with cisplatin is synergistic. It was also observed that epicatechin promotes cell death by apoptosis. Conclusions: Epicatechin might be considered for future studies to explore its possible use as coadjuvant in cisplatin-based treatments. |
| format |
Article |
| author |
Varela-Castillo, O. Cordero, P. Gutiérrez-Iglesias, G. Palma, I. Rubio-Gayosso, I. Meaney, E. Ramirez-Sanchez, I. Villarreal, F. Ceballos, G. Nájera, N. |
| author_facet |
Varela-Castillo, O. Cordero, P. Gutiérrez-Iglesias, G. Palma, I. Rubio-Gayosso, I. Meaney, E. Ramirez-Sanchez, I. Villarreal, F. Ceballos, G. Nájera, N. |
| author_sort |
Varela-Castillo, O. |
| title |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach |
| title_short |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach |
| title_full |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach |
| title_fullStr |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach |
| title_full_unstemmed |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach |
| title_sort |
characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line a549. an isobolographic approach |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2018 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139247 |
| citation_txt |
Characterization of the cytotoxic effects of the combination of cisplatin and flavanol (-)-epicatechin on human lung cancer cell line A549. An isobolographic approach / O. Varela-Castillo, P. Cordero, G. Gutiérrez-Iglesias, I. Palma, I. Rubio-Gayosso, E. Meaney, I. Ramirez-Sanchez, F. Villarreal, G. Ceballos, N. Nájera // Experimental Oncology. — 2018 — Т. 40, № 1. — С. 19-23. — Бібліогр.: 25 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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| first_indexed |
2023-10-18T21:19:23Z |
| last_indexed |
2023-10-18T21:19:23Z |
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