The effects of antioxidants on diabetic damage with a tobacco smoke in bovine lenses

• Видавець: Фізико-хімічний інститут ім. О.В. Богатського НАН України
• Дата: 2014
• Автори: Bormusov, E.A., Reznick, A.Z.
• Формат: Стаття
• Мова: English
• Опубліковано: Фізико-хімічний інститут ім. О.В. Богатського НАН України 2014
• Назва видання: Актуальні проблеми транспортної медицини
• Теми: Экспериментальные исследования
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/139505
• Цитувати: The effects of antioxidants on diabetic damage with a tobacco smoke in bovine lenses / E.A. Bormusov, A.Z. Reznick // Актуальні проблеми транспортної медицини. — 2014. — № 3 (37). — С. 96-109. — Бібліогр.: 24 назв. — англ.

Investigated the mechanisms involved in the effects of preventing cataract and cigarette smoking (CS) when exposed to the lens antioxidants. Bovine lenses were removed in organ culture for 12 days, exposed to glucose 450 mg % for antioxidants including-Desferioxamine (DFO) and cysteine N-acetyl-L-(NAC). Treated lenses were 4 days in culture medium saturated with cigarette smoke daily dose at 500 psi. The use of laser-lens optical quality was assessed daily. At the end of the incubation period, lenses were analyzed using an inverted microscopy as epithelial layer was used for histochemical assessment method Einarson nucleic acids -RNA-DNA staining. Reactive Oxygen Species (ROS) were evaluated C6827, to measure the level of cellular oxidation in the epithelial cells of the lens relative to the control cultures by fluorescence microscopy. High glucose with a smoke causes optical and morphological changes in epithelial cells (hypertrophy, hyperplasia). Antioxidants reduce the damage caused by high glucose and CS, to protect the lens from high glucose, reduce cell damage, prevent the increased activity of ROS. NAC protected the lens from damage high glucose better than DFO. We suggest that NAC can serve as an effective advocate for the lens of the eye against damage from smoking diabetics.