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Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy

Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy

To investigate properties of leukemic cells by sorting out children diagnosed with ALL with different response to chemotherapy based on MRD level. Methods: We used a minimal residual disease (MRD) data on day 36 obtained with 3-colour flow cytometry as a reference. In view of MRD results, we used re...

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Main Authors: Fedasenka, U.U., Shman, T.V., Savitski, V.P., Belevcev, M.V.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
Series:Experimental Oncology
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Online Access:http://dspace.nbuv.gov.ua/handle/123456789/139903
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spelling irk-123456789-1399032018-06-22T03:04:51Z Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy Fedasenka, U.U. Shman, T.V. Savitski, V.P. Belevcev, M.V. Uncategorized To investigate properties of leukemic cells by sorting out children diagnosed with ALL with different response to chemotherapy based on MRD level. Methods: We used a minimal residual disease (MRD) data on day 36 obtained with 3-colour flow cytometry as a reference. In view of MRD results, we used real-time PCR to assess expression levels of multidrug resistance associated genes MDR1, LRP and BCRP, antiapoptotic gene Bcl-2 in initial samples from children diagnosed with ALL. P-gp expression and function in initial samples were analyzed by flow cytometry. Results: Briefly, medians of relative expression levels of MDR1 gene were roughly comparable and in MRD+ group came to 22.8 (0.02–26.6; n = 9) vs 24.8 (3.9–41.4; n = 10) in MRD– group. Bcl-2 gene showed tendency to higher expression levels in MRD+ group with median at 5992.9 (521.0–10362.0; n = 9) compared to 3183.6 (1947.9–6581.0; n = 10) in MRD– group. LRP gene relative expression levels were similar in both groups and came to 1934.9 (1500.7–3490.4; n = 9) and 1408.5 (665.5–2917.1; n = 10) in MRD+ and MRD– groups, respectively. The median of BCRP expression levels in MRD+ group was considerably lower than that in MRD– group, namely 76000.0 (48196.2–169230.8; n = 9) and 227967.2 (16683.7–422222.2; n = 10), respectively, but statistical analysis showed no significant difference for this parameter. Conclusion: We investigated expression of multidrug resistance genes MDR1, LRP and BCRP and antiapoptotic gene Bcl-2 in leukemic cells at diagnosis, and MRD level at the end of induction therapy, and could not find obvious relations between these parameters. Цель: изучить свойства лейкозных клеток детей больных ОЛЛ с положительным и отрицательным результатом мониторинга минимальной резидуальной болезни после индукционной терапии. Методы: согласно результатам мониторинга минимальной резидуальной болезни (MRD) с помощью трехцветной проточной цитометрии на 36-й день больные были разделены на 2 группы: MRD– и MRD+. Для изучения уровней экспрессии генов MDR1, LRP, BCRP и BCL-2 в образцах костного мозга пациентов с диагнозом первичного ОЛЛ использовался метод относительной количественной ПЦР в режиме реального времени. Результаты: медианы уровней экспрессии гена MDR1 в изученных группах пациентов отличались незначительно и составили 22,8 (0,02–26,6; n = 9) в MRD+ и 24,8 (3,9–41,4; n = 10) в MRD–-группе. Тенденция к повышенной экспрессии гена BCL-2 выявлена в группе MRD+, где медиана составила 5992,9 (521,0–10362,0; n = 9), по сравнению с 3183,6 (1947,9–6581,0; n = 10) в группе MRD–. Относительная экспрессия гена LRP в изучаемых группах оказалась сходной, медианы составили 1934,9 (1500,7–3490,4; n = 9) и 1408,5 (665,5–2917,1; n = 10) в MRD+- и MRD–-группах соответственно. Медиана уровня экспрессии гена BCRP в группе MRD+ была значительно ниже таковой в группе MRD– — 76000,0 (48196,2–169230,8; n = 9) и 227967,2 (16683,7–422222,2; n = 10) соответственно, однако статистических анализ не подтвердил значимость различий. Выводы: при изучении уровней экспрессии генов MDR1, LRP, BCRP и BCL-2 в лейкозных клетках при установлении первичного диагноза ОЛЛ у детей и при мониторинге MRD после индукционной терапии не выявлена связь между данными параметрами. 2008 Article Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy / U.U. Fedasenka, T.V. Shman, V.P. Savitski, M.V. Belevcev // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 248–252. — Бібліогр.: 51 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139903 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Uncategorized
Uncategorized
spellingShingle Uncategorized
Uncategorized
Fedasenka, U.U.
Shman, T.V.
Savitski, V.P.
Belevcev, M.V.
Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
Experimental Oncology
description To investigate properties of leukemic cells by sorting out children diagnosed with ALL with different response to chemotherapy based on MRD level. Methods: We used a minimal residual disease (MRD) data on day 36 obtained with 3-colour flow cytometry as a reference. In view of MRD results, we used real-time PCR to assess expression levels of multidrug resistance associated genes MDR1, LRP and BCRP, antiapoptotic gene Bcl-2 in initial samples from children diagnosed with ALL. P-gp expression and function in initial samples were analyzed by flow cytometry. Results: Briefly, medians of relative expression levels of MDR1 gene were roughly comparable and in MRD+ group came to 22.8 (0.02–26.6; n = 9) vs 24.8 (3.9–41.4; n = 10) in MRD– group. Bcl-2 gene showed tendency to higher expression levels in MRD+ group with median at 5992.9 (521.0–10362.0; n = 9) compared to 3183.6 (1947.9–6581.0; n = 10) in MRD– group. LRP gene relative expression levels were similar in both groups and came to 1934.9 (1500.7–3490.4; n = 9) and 1408.5 (665.5–2917.1; n = 10) in MRD+ and MRD– groups, respectively. The median of BCRP expression levels in MRD+ group was considerably lower than that in MRD– group, namely 76000.0 (48196.2–169230.8; n = 9) and 227967.2 (16683.7–422222.2; n = 10), respectively, but statistical analysis showed no significant difference for this parameter. Conclusion: We investigated expression of multidrug resistance genes MDR1, LRP and BCRP and antiapoptotic gene Bcl-2 in leukemic cells at diagnosis, and MRD level at the end of induction therapy, and could not find obvious relations between these parameters.
format Article
author Fedasenka, U.U.
Shman, T.V.
Savitski, V.P.
Belevcev, M.V.
author_facet Fedasenka, U.U.
Shman, T.V.
Savitski, V.P.
Belevcev, M.V.
author_sort Fedasenka, U.U.
title Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
title_short Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
title_full Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
title_fullStr Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
title_full_unstemmed Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy
title_sort expression of mdr1, lrp, bcrp and bcl-2 genes at diagnosis of childhood all: comparison with mrd status after induction therapy
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2008
topic_facet Uncategorized
url http://dspace.nbuv.gov.ua/handle/123456789/139903
citation_txt Expression of MDR1, LRP, BCRP and BCL-2 genes at diagnosis of childhood ALL: comparison with mrd status after induction therapy / U.U. Fedasenka, T.V. Shman, V.P. Savitski, M.V. Belevcev // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 248–252. — Бібліогр.: 51 назв. — англ.
series Experimental Oncology
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