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Proteomic approaches in biological and medical sciences: principles and applications

Proteomic approaches in biological and medical sciences: principles and applications

After the first introduction of the concept of “proteome” more than 10 years ago, large-scale studies of protein expression, localization, activities and interactions have gained an exponential increase of interest, leading to extensive research and technology development. Proteomics is expansively...

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Main Authors: Conrotto, P., Souchelnytskyi, S.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
Series:Experimental Oncology
Subjects:
Reviews
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/139904
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spelling irk-123456789-1399042018-06-22T03:08:14Z Proteomic approaches in biological and medical sciences: principles and applications Conrotto, P. Souchelnytskyi, S. Reviews After the first introduction of the concept of “proteome” more than 10 years ago, large-scale studies of protein expression, localization, activities and interactions have gained an exponential increase of interest, leading to extensive research and technology development. Proteomics is expansively applied in many areas, ranging from basic research, various disease and malignant tumors diagnostic and biomarker discovery to therapeutic applications. Several proteomics approaches have been developed for protein separation and identification, and for the characterization of protein function and structure. Two-dimensional gel electrophoresis, chromatography, capillary electrophoresis and mass spectrometry have become the most used proteomics methods. These techniques are also under constant development. This review provides an overview of the main techniques and their combinations, used in proteomics. The emphasis is made on description of advantages and disadvantages of each technique, to navigate in selection of the best application for solving a specific problem. Развитие протеомики за последние 10 лет стимулировало возрастающий интерес к ее применению для решения важных проблем современной биологии и медицины. Сегодня протеомика активно используется в фундаментальных исследованиях, поиске биомаркеров, диагностике опухолей. Широкомасштабное изучение экспрессии, локализации, активности и взаимодействий белков привело к специализации протеомных технологий и методов. Поэтому вопрос подбора наиболее подходящих методов для решения специфических проблем является особо актуальным. Двухмерный электрофорез, жидкостная хроматография, капиллярный электрофорез и масс-спектрометрия являются наиболее развитыми технологиями протеомики. В этом обзоре проанализированы сильные и слабые стороны этих технологий. Авторы надеются, что этот анализ поможет читателям выбрать наиболее эффективную методику для решения соответствующих научных задач. 2008 Article Proteomic approaches in biological and medical sciences: principles and applications / P. Conrotto, S. Souchelnytskyi // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 171–180. — Бібліогр.: 55 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139904 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Reviews
Reviews
spellingShingle Reviews
Reviews
Conrotto, P.
Souchelnytskyi, S.
Proteomic approaches in biological and medical sciences: principles and applications
Experimental Oncology
description After the first introduction of the concept of “proteome” more than 10 years ago, large-scale studies of protein expression, localization, activities and interactions have gained an exponential increase of interest, leading to extensive research and technology development. Proteomics is expansively applied in many areas, ranging from basic research, various disease and malignant tumors diagnostic and biomarker discovery to therapeutic applications. Several proteomics approaches have been developed for protein separation and identification, and for the characterization of protein function and structure. Two-dimensional gel electrophoresis, chromatography, capillary electrophoresis and mass spectrometry have become the most used proteomics methods. These techniques are also under constant development. This review provides an overview of the main techniques and their combinations, used in proteomics. The emphasis is made on description of advantages and disadvantages of each technique, to navigate in selection of the best application for solving a specific problem.
format Article
author Conrotto, P.
Souchelnytskyi, S.
author_facet Conrotto, P.
Souchelnytskyi, S.
author_sort Conrotto, P.
title Proteomic approaches in biological and medical sciences: principles and applications
title_short Proteomic approaches in biological and medical sciences: principles and applications
title_full Proteomic approaches in biological and medical sciences: principles and applications
title_fullStr Proteomic approaches in biological and medical sciences: principles and applications
title_full_unstemmed Proteomic approaches in biological and medical sciences: principles and applications
title_sort proteomic approaches in biological and medical sciences: principles and applications
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2008
topic_facet Reviews
url http://dspace.nbuv.gov.ua/handle/123456789/139904
citation_txt Proteomic approaches in biological and medical sciences: principles and applications / P. Conrotto, S. Souchelnytskyi // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 171–180. — Бібліогр.: 55 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT conrottop proteomicapproachesinbiologicalandmedicalsciencesprinciplesandapplications
AT souchelnytskyis proteomicapproachesinbiologicalandmedicalsciencesprinciplesandapplications
first_indexed 2023-10-18T21:21:24Z
last_indexed 2023-10-18T21:21:24Z
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