Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma

Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma

To investigate the effect of teichoic acid (TA) from the cell wall of S. aureus on some indices of immunological reactivity in mice bearing Lewis lung carcinoma (LLC). Methods: The teichoic acid at the doses of 1, 2 and 4 µg/g of body weight has been administered subcutaneously simultaneously with t...

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Дата:2008
Автори: Potebnya, G.P., Skivka, L.M., Pozur, V.V., Rudik, M.P., Senchilo, N.V., Fedorchuk, O.G., Khranovska, N.M.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
Назва видання:Experimental Oncology
Теми:
Uncategorized
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/139906
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Influence of teichoic acid from S. aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma / G.P. Potebnya, L.M. Skivka, V.V. Pozur, M.P. Rudik, N.V. Senchilo, O.G. Fedorchuk, N.M. Khranovska // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–223. — Бібліогр.: 28 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1399062018-06-22T03:03:54Z Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma Potebnya, G.P. Skivka, L.M. Pozur, V.V. Rudik, M.P. Senchilo, N.V. Fedorchuk, O.G. Khranovska, N.M. Uncategorized To investigate the effect of teichoic acid (TA) from the cell wall of S. aureus on some indices of immunological reactivity in mice bearing Lewis lung carcinoma (LLC). Methods: The teichoic acid at the doses of 1, 2 and 4 µg/g of body weight has been administered subcutaneously simultaneously with tumor cells transplantation and in 7 days. The cytotoxic activity of peritoneal macrophages has been assessed by NBT-test. The splenocyte cytotoxic activity against the LLC cells has been tested by flow cytometry. The evaluation of tumor infiltration by lymphoid cells was carried out as well. Results: TA had no significant effect on oxidative metabolism of peritoneal macrophages in tumor bearing mice. Upon TA administration, the cytotoxic activity of splenocytes against the LLC cells has been augmented in a dose-dependent manner (at the TA dose of 4 µg/g, 2-fold decrease of tumor growth and metastasis has been registered) and leads to decreased tumor infiltration by mononuclear cells. Conclusion: TA caused a dose dependent inhibition of growth and metastasis of LLC. It was supposed that TA can influence the tumor grows by activation of splenocytes cytotoxic activity. Цель: исследовать влияние тейхоевой кислоты Staphylococcus aureus Wood 46 (ТК) на иммунологические параметры у мышей с карциномой легкого Льюис. Методы: в качестве модели использовали перевиваемую карциному легкого Льюис. Тейхоевую кислоту S. aureus Wood 46 получали по методу Арчибальда. Кислородзависимый метаболизм перитонеальных макрофагов оценивали по восстановлению нитросинего тетразолия (НСТ-тест). Цитотоксическую активность спленоцитов определяли методом проточной цитофлуориметрии. Результаты: ТК оказывала дозозависимое влияние на рост и метастазирование карциномы Льюис. У животных, получивших ТК в дозе 4 мкг/г, размеры первичной опухоли были на момент окончания эксперимента в 4 разa меньше по сравнению с контролем, метастазы в легких отсутствовали. ТК незначительно стимулировала кислородзависимый метаболизм перитонеальных макрофагов. Отмечали дозозависимую активацию цитотоксической активности спленоцитов: при использовании ТК в дозе 4 мкг/г цитотоксическая активность спленоцитов у животных опытной группы в 2 раза превышала показатели контрольных животных-носителей опухолей. Выводы: ТК оказывает дозозависимое ингибиторное влияние на рост и метастазирование карциномы легкого Льюис. Одним из механизмов, опосредующих супрессорное действие ТК на опухолевый рост, можнo считать активацию цитотоксической активности спленоцитов. 2008 Article Influence of teichoic acid from S. aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma / G.P. Potebnya, L.M. Skivka, V.V. Pozur, M.P. Rudik, N.V. Senchilo, O.G. Fedorchuk, N.M. Khranovska // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–223. — Бібліогр.: 28 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139906 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Uncategorized
Uncategorized
spellingShingle Uncategorized
Uncategorized
Potebnya, G.P.
Skivka, L.M.
Pozur, V.V.
Rudik, M.P.
Senchilo, N.V.
Fedorchuk, O.G.
Khranovska, N.M.
Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
Experimental Oncology
description To investigate the effect of teichoic acid (TA) from the cell wall of S. aureus on some indices of immunological reactivity in mice bearing Lewis lung carcinoma (LLC). Methods: The teichoic acid at the doses of 1, 2 and 4 µg/g of body weight has been administered subcutaneously simultaneously with tumor cells transplantation and in 7 days. The cytotoxic activity of peritoneal macrophages has been assessed by NBT-test. The splenocyte cytotoxic activity against the LLC cells has been tested by flow cytometry. The evaluation of tumor infiltration by lymphoid cells was carried out as well. Results: TA had no significant effect on oxidative metabolism of peritoneal macrophages in tumor bearing mice. Upon TA administration, the cytotoxic activity of splenocytes against the LLC cells has been augmented in a dose-dependent manner (at the TA dose of 4 µg/g, 2-fold decrease of tumor growth and metastasis has been registered) and leads to decreased tumor infiltration by mononuclear cells. Conclusion: TA caused a dose dependent inhibition of growth and metastasis of LLC. It was supposed that TA can influence the tumor grows by activation of splenocytes cytotoxic activity.
format Article
author Potebnya, G.P.
Skivka, L.M.
Pozur, V.V.
Rudik, M.P.
Senchilo, N.V.
Fedorchuk, O.G.
Khranovska, N.M.
author_facet Potebnya, G.P.
Skivka, L.M.
Pozur, V.V.
Rudik, M.P.
Senchilo, N.V.
Fedorchuk, O.G.
Khranovska, N.M.
author_sort Potebnya, G.P.
title Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
title_short Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
title_full Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
title_fullStr Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
title_full_unstemmed Influence of teichoic acid from S. Aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma
title_sort influence of teichoic acid from s. aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing lewis lung carcinoma
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2008
topic_facet Uncategorized
url http://dspace.nbuv.gov.ua/handle/123456789/139906
citation_txt Influence of teichoic acid from S. aureus on metabolic activity of macrophages and cytotoxic activity of splenocytes of mice bearing Lewis lung carcinoma / G.P. Potebnya, L.M. Skivka, V.V. Pozur, M.P. Rudik, N.V. Senchilo, O.G. Fedorchuk, N.M. Khranovska // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–223. — Бібліогр.: 28 назв. — англ.
series Experimental Oncology
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first_indexed 2023-10-18T21:21:24Z
last_indexed 2023-10-18T21:21:24Z
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