Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma
Porphyrins and new chitosan hydrogels based composites with porphyrins are used as active cytotoxic antitumor agents in photodynamic therapy (PDT). Aim: The present study evaluates the effects of photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) and 5-ALA associated with chitosan (CS) us...
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| Дата: | 2008 |
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| Автори: | , , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2008
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/139910 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma / A. Filip, S. Clichici, A. Muresan, D. Daicoviciu, C. Tatomir, C. Login, S. Dreve, C. Gherman // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–219. — Бібліогр.: 32 назв. — англ. |
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irk-123456789-1399102018-06-22T03:03:57Z Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma Filip, A. Clichici, S. Muresan, A. Daicoviciu, D. Tatomir, C. Login, C. Dreve, S. Gherman, C. Uncategorized Porphyrins and new chitosan hydrogels based composites with porphyrins are used as active cytotoxic antitumor agents in photodynamic therapy (PDT). Aim: The present study evaluates the effects of photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) and 5-ALA associated with chitosan (CS) using Walker carcinosarcoma in rats as experimental model. Methods: The animals were irradiated with red light (l = 685 nm, D = 50 J/cm2, 15 min) 3 h after i.p. administration of 5-ALA (250 mg/kg b.w.) or a mixture of 5-ALA (250 mg/kg b.w.) and CS (1.5 mg/kg b.w.). The animals were sacrificed at 1, 3, 6, 24 h and 14 days after the treatment. The effects of PDT were investigated by morphological studies, monitoring the 5-ALA induced protoporphyrin IX (Pp IX) level in tumor tissue and serum, MMP 2 and 9 (gelatinases) activity in tumor and malondialdehyde level (MDA), marker of the lipoperoxidation process, in tumor and serum. Results: Zymography revealed an increased activity of MMP 2 in tumors from animals treated with 5-ALA PDT. PDT with 5-ALA induced a higher lipid peroxidation in tumor tissue compared with 5-ALA-CS. CS associated to 5 ALA PDT enhanced the accumulation of PS in tumors inducing earlier necrotic changes. In the same time CS reduced MMP 2 activity. Conclusion: Our results suggest that MMPs activation and oxygen reactive species are involved in PDT effects. Порфирины и новые соединения, основу которых составляют гидрогели хитозана с порфиринами, используются как активные цитотоксические противоопухолевые препараты при фотодинамической терапии (PDT). Цель: оценить действие PDT с 5-аминолевуленовой кислотой (5-ALA) и 5-ALA, ассоциированной с хитозаном (CS), на клетки карциносаркомы Уокера. Методы: крыс облучали красным светом (λ = 685 нм, D = 50 Дж/см2 , 15 мин) 3 ч после внутрибрюшинного введения 5-ALA (250 мг/кг) или смеси 5-ALA (250 мг/кг) и CS (1,5 мг/кг). Подопытных животных забивали через 1 ч, 3 ч, 6 ч, 24 ч и 14 дней после воздействия PDT. Эффект PDT определяли с помощью морфологических исследований, регистрируя уровень протопорфирина IX (Pp IX), вызываемого 5-ALA, в опухолевой ткани и сыворотке крови, активность MMP 2 и 9 (желатиназы) в опухоли и уровень малонового диальдегида (MDA), маркера процесса перекисного окисления липидов, в опухоли и сыворотке крови. Результаты: зимографические исследования показали повышенную активность MMP 2 в опухолях животных, которых подвергали 5-ALA PDT. PDT с 5-ALA вызывала повышенный уровень перекисного окисления липидов в опухолевой ткани по сравнению с 5-ALA-CS. CS с 5 ALA PDT усиливал накопление фотосенсибилизирующего вещества (PS) в опухолях, вызывая более ранние некротические изменения. В то же время CS снижал активность MMP 2. Выводы: полученные результаты позволяют предположить, что для проявления эффектов PDT необходимы активация MMP и образование активных форм кислорода. 2008 Article Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma / A. Filip, S. Clichici, A. Muresan, D. Daicoviciu, C. Tatomir, C. Login, S. Dreve, C. Gherman // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–219. — Бібліогр.: 32 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/139910 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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English |
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Uncategorized Uncategorized |
| spellingShingle |
Uncategorized Uncategorized Filip, A. Clichici, S. Muresan, A. Daicoviciu, D. Tatomir, C. Login, C. Dreve, S. Gherman, C. Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma Experimental Oncology |
| description |
Porphyrins and new chitosan hydrogels based composites with porphyrins are used as active cytotoxic antitumor agents in photodynamic therapy (PDT). Aim: The present study evaluates the effects of photodynamic therapy (PDT) with 5-aminolevulinic acid (5-ALA) and 5-ALA associated with chitosan (CS) using Walker carcinosarcoma in rats as experimental model. Methods: The animals were irradiated with red light (l = 685 nm, D = 50 J/cm2, 15 min) 3 h after i.p. administration of 5-ALA (250 mg/kg b.w.) or a mixture of 5-ALA (250 mg/kg b.w.) and CS (1.5 mg/kg b.w.). The animals were sacrificed at 1, 3, 6, 24 h and 14 days after the treatment. The effects of PDT were investigated by morphological studies, monitoring the 5-ALA induced protoporphyrin IX (Pp IX) level in tumor tissue and serum, MMP 2 and 9 (gelatinases) activity in tumor and malondialdehyde level (MDA), marker of the lipoperoxidation process, in tumor and serum. Results: Zymography revealed an increased activity of MMP 2 in tumors from animals treated with 5-ALA PDT. PDT with 5-ALA induced a higher lipid peroxidation in tumor tissue compared with 5-ALA-CS. CS associated to 5 ALA PDT enhanced the accumulation of PS in tumors inducing earlier necrotic changes. In the same time CS reduced MMP 2 activity. Conclusion: Our results suggest that MMPs activation and oxygen reactive species are involved in PDT effects. |
| format |
Article |
| author |
Filip, A. Clichici, S. Muresan, A. Daicoviciu, D. Tatomir, C. Login, C. Dreve, S. Gherman, C. |
| author_facet |
Filip, A. Clichici, S. Muresan, A. Daicoviciu, D. Tatomir, C. Login, C. Dreve, S. Gherman, C. |
| author_sort |
Filip, A. |
| title |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma |
| title_short |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma |
| title_full |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma |
| title_fullStr |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma |
| title_full_unstemmed |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma |
| title_sort |
efects of pdt with 5-aminolevulinic acid and chitosan on walker carcinosarcoma |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2008 |
| topic_facet |
Uncategorized |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/139910 |
| citation_txt |
Efects of PDT with 5-aminolevulinic acid and chitosan on Walker carcinosarcoma / A. Filip, S. Clichici, A. Muresan, D. Daicoviciu, C. Tatomir, C. Login, S. Dreve, C. Gherman // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 212–219. — Бібліогр.: 32 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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2023-10-18T21:21:25Z |
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2023-10-18T21:21:25Z |
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