Impact of IFN-g gene polimorphism on the risk of cervical cancer

• Дата: 2008
• Автори: Kordi Tamandani, M.K., Sobti, R.C., Shekari, M., Suri, V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2008
• Назва видання: Experimental Oncology
• Теми: Uncategorized
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/139922
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Impact of IFN-g gene polimorphism on the risk of cervical cancer / M.K. Kordi Tamandani, R.C. Sobti, M. Shekari, M. Mukesh, V. Suri // Experimental Oncology. — 2008. — Т. 30, № 3. — С. 224–229. — Бібліогр.: 35 назв. — англ.

Cervical cancer, the second most common malignancy in women worldwide, is almost invariably associated with infection by human papillomavirus (HPV). However, although many women are infected with high-risk types of HPV, only a subset of infected women will ever develop cervical cancer. Several studies suggested that immunological components play a key role in the development of cervical cancer. Interferon gamma (IFN-g) is a cytokine produced by activated T cells and natural killer (NK) cells that enhances cellular immune responses by increasing T-cell cytotoxicity and NK cell activity. Aim: To study single nucleotide polymorphism (SNP), T to A, located at the +874 position and measure IFN-g messenger RNA (mRNA) at the tumor site. Methods: DNA was isolated from peripheral blood of 200 patients with cervical cancer and 200 healthy controls. The allele polymorphism at position +874 in the IFN-g gene was studied by ARMS-PCR (Amplification Refractory Mutation System) and measured IFN-g mRNA at the tumor site by means of a semi-quantitative polymerase chain reaction (sqRT-PCR) assay. Results: It was observed that genotypes AT and AA + AT increase the risk of cervical cancer (OR = 3.3, 95% CI — 2.05–5.2, P ≤ 0.001 — OR = 2.9, 95% CI — 1.9–4.6, P ≤ 0.001, respectively). In case of passive smokers same genotypes showed highly significant increased risk of cervical cancer (OR = 5.55, 95% CI = 2.77–11.19 — OR = 5.25, 95% CI = 2.77–10, respectively). Thus, the sqRT-PCR reflected the similar level of mRNA expression of IFN-g gene in patients suffering from cervical carcinoma and healthy controls. Conclusion: This is the first study to provide an evidence for effecting of IFN-g gene on the risk of cervical cancer in north Indian population.