Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus

• Дата: 2016
• Автори: Lykhova, O., Kovalova, O., Bezdenezhnykh, N., Adamenko, I., Vorontsova, A., Strokovska, L., Kudryavets, Yu.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2016
• Назва видання: Experimental Oncology
• Теми: Original contributions
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/140132
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ.

Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed expression ofproteins associated with EMT.