Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus
Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lu...
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| Дата: | 2016 |
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| Автори: | , , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2016
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/140132 |
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| Цитувати: | Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. |
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irk-123456789-1401322018-06-23T03:03:13Z Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. Original contributions Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed expression ofproteins associated with EMT. 2016 Article Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/140132 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus Experimental Oncology |
| description |
Aim: To analyze biological characteristics. malignant potential and expression of proteins associated with epithelial mesenchynal
transition in murine lung carclnoma cells transduced with intertcron-beta (ifn-β) gene in baculovirus vector. Materials and Methods: The study was perforated on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector.
Biological characterlstics of the LL cells were studied with the use of standard cell culture method, cytogenetic and immunocytochemical assays. Resutes: Recombinant baculmirus-mediated transduction of LL cells with ifn-β gene resulted in siguficant decrease
of cell growth rate and density both in complete and serum-foee medium. Also. LL cells transduction with ifn-β gene significantly
inhfltited cell migration in vitro. Transduction of LL cells by baculovirus vector with or withoot ifn-β gene caused significant genotoxic effect in these cells. Furthermore. ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19ARE (p < 0.01). p21WAFI (p < 0.001). cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription
factors of epithelial-mesenchymal transition (EMT) Tmist (p < 0.005) and Slug (p < 0.00 I) expression. Conclusions; Transduction
with ifn-β gene of LL cells in recombinant baculovirus resulted in aquirement of less malignant phenotype in vitro and suppressed
expression ofproteins associated with EMT. |
| format |
Article |
| author |
Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. |
| author_facet |
Lykhova, O. Kovalova, O. Bezdenezhnykh, N. Adamenko, I. Vorontsova, A. Strokovska, L. Kudryavets, Yu. |
| author_sort |
Lykhova, O. |
| title |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_short |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_full |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_fullStr |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_full_unstemmed |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| title_sort |
inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2016 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/140132 |
| citation_txt |
Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus / O. Lykhova, O. Kovalova, N. Bezdenezhnykh, I. Adamenko, A. Vorontsova, L. Strokovska, Yu. Kudryavets // Experimental Oncology. — 2016 — Т. 38, № 1. — С. 13-21. — Бібліогр.: 37 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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| first_indexed |
2023-10-18T21:22:07Z |
| last_indexed |
2023-10-18T21:22:07Z |
| _version_ |
1796152626588418048 |