Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype
Aim:The aim of our study was to determine the complex of molecular genetic markers which are associated with cancer aggressiveness, invasion and metastasis among different molecular subtypes of breast cancer cell lines. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7,...
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| Дата: | 2013 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2013
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| Назва видання: | Experimental Oncology |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145247 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype / S. Chekhun, N. Bezdenezhnykh, J. Shvets, N. Lukianova // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 174-179. — Бібліогр.: 41 назв. — англ. |
Репозитарії
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irk-123456789-1452472019-01-20T01:23:30Z Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype Chekhun, S. Bezdenezhnykh, N. Shvets, J. Lukianova, N. Original contributions Aim:The aim of our study was to determine the complex of molecular genetic markers which are associated with cancer aggressiveness, invasion and metastasis among different molecular subtypes of breast cancer cell lines. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A and 184A1. Expression of estrogen receptor, progesterone receptor, Her-2/neu and Ki-67 was studied by immunocytochemical method. CD24, CD44 and E-cadherin expression was studied by flow cytometry. Results: We have identified biomarkers which characterize metastatic potential of human breast cancer cells of certain molecular subtypes. It has been demonstrated that low colony forming activity of human breast cancer cells of luminal subtype is accompanied by increased adhesive properties of these cells due to high level of E-cadherin expression, low level of CD44 expression and absence of CD24 expression. High tumorigenicity of cells of basal subtype is connected to weakening of adhesive contacts that is caused by abnormalities of E-cadherin expression, significant increase of CD44 expression and presence of low level of CD24 expression. Conclusion: Our data indicated that changes of correlation between expression of cellular adhesion molecules inside conventional immunohistochemical subtypes reflect significantly wider biological properties of luminal and basal subtypes of human breast cancer. 2013 Article Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype / S. Chekhun, N. Bezdenezhnykh, J. Shvets, N. Lukianova // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 174-179. — Бібліогр.: 41 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145247 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Chekhun, S. Bezdenezhnykh, N. Shvets, J. Lukianova, N. Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype Experimental Oncology |
| description |
Aim:The aim of our study was to determine the complex of molecular genetic markers which are associated with cancer aggressiveness, invasion and metastasis among different molecular subtypes of breast cancer cell lines. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A and 184A1. Expression of estrogen receptor, progesterone receptor, Her-2/neu and Ki-67 was studied by immunocytochemical method. CD24, CD44 and E-cadherin expression was studied by flow cytometry. Results: We have identified biomarkers which characterize metastatic potential of human breast cancer cells of certain molecular subtypes. It has been demonstrated that low colony forming activity of human breast cancer cells of luminal subtype is accompanied by increased adhesive properties of these cells due to high level of E-cadherin expression, low level of CD44 expression and absence of CD24 expression. High tumorigenicity of cells of basal subtype is connected to weakening of adhesive contacts that is caused by abnormalities of E-cadherin expression, significant increase of CD44 expression and presence of low level of CD24 expression. Conclusion: Our data indicated that changes of correlation between expression of cellular adhesion molecules inside conventional immunohistochemical subtypes reflect significantly wider biological properties of luminal and basal subtypes of human breast cancer. |
| format |
Article |
| author |
Chekhun, S. Bezdenezhnykh, N. Shvets, J. Lukianova, N. |
| author_facet |
Chekhun, S. Bezdenezhnykh, N. Shvets, J. Lukianova, N. |
| author_sort |
Chekhun, S. |
| title |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| title_short |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| title_full |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| title_fullStr |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| title_full_unstemmed |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| title_sort |
expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2013 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145247 |
| citation_txt |
Expression of biomarkers related to cell adhesion, metastasis and invasion of breast cancer cell lines of different molecular subtype / S. Chekhun, N. Bezdenezhnykh, J. Shvets, N. Lukianova // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 174-179. — Бібліогр.: 41 назв. — англ. |
| series |
Experimental Oncology |
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AT chekhuns expressionofbiomarkersrelatedtocelladhesionmetastasisandinvasionofbreastcancercelllinesofdifferentmolecularsubtype AT bezdenezhnykhn expressionofbiomarkersrelatedtocelladhesionmetastasisandinvasionofbreastcancercelllinesofdifferentmolecularsubtype AT shvetsj expressionofbiomarkersrelatedtocelladhesionmetastasisandinvasionofbreastcancercelllinesofdifferentmolecularsubtype AT lukianovan expressionofbiomarkersrelatedtocelladhesionmetastasisandinvasionofbreastcancercelllinesofdifferentmolecularsubtype |
| first_indexed |
2023-05-20T17:21:38Z |
| last_indexed |
2023-05-20T17:21:38Z |
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