Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine

Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine

Aim: The aim of this study was to examine the JAK2 V617F, the G1691A allele of factor V, and the G20210A prothrombin gene mutation status, and their predictive value for thrombosis in patients with Ph-negative myeloproliferative neoplasms (MPN) in Ukraine, with special emphasize to patient exposed t...

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Дата:2013
Автори: Mishcheniuk, O.Y., Kostukevich, O.M., Dmytrenko, I.V., Sholoyko, V.V., Prokopenko, I.M., Martіna, Z.V., Pilipenko, G.V., Klymenko, S.V.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2013
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145251
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine / O.Y. Mishcheniuk, O.M. Kostukevich, I.V. Dmytrenko, V.V. Sholoyko, I.M. Prokopenko, Z.V. Martіna, G.V. Pilipenko, S.V. Klymenko // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 202-206. — Бібліогр.: 22 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1452512019-01-20T01:23:27Z Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine Mishcheniuk, O.Y. Kostukevich, O.M. Dmytrenko, I.V. Sholoyko, V.V. Prokopenko, I.M. Martіna, Z.V. Pilipenko, G.V. Klymenko, S.V. Original contributions Aim: The aim of this study was to examine the JAK2 V617F, the G1691A allele of factor V, and the G20210A prothrombin gene mutation status, and their predictive value for thrombosis in patients with Ph-negative myeloproliferative neoplasms (MPN) in Ukraine, with special emphasize to patient exposed to ionizing radiation due to the Chernobyl accident. Materials and Methods: There were 198 patients with Ph-negative MPN included in the study. Of these, 45 patients had experienced radiation exposure due to the Chernobyl accident. The JAK2 V617F mutation, the G1691A of factor V and the G20210A of prothrombin were detected by allele-specific polymerase chain reaction. Results: The polycythemia vera and essential thrombocythemia patients in unexposed group and Chernobyl patients were comparable in terms of the JAK2 V617F mutation prevalence with the frequency of anomaly corresponding well to the published data on unselected cases of these types of Ph-negative MPN. The JAK2 V617F mutation was less common on the border of statistical significance (p = 0.08) in Chernobyl primary myelofibrosis (PMF) patients than in non-exposed patients. JAK2 V617F positive patients had higher level of leukocytes (p = 0.03), hemoglobin (p =0.04) and splenomegaly (p = 0.04) than those without mutation. The JAK2 V617F mutation was strong predictor for thrombosis in essential thrombocytemia patients (relative risk=3.1, 95% CI = 1.7–16.4, p = 0.03). In PMF, the association with thrombosis was found for the G1691A allele of factor V (p = 0.03). The risk of thrombosis associated with the inherited thrombophilia in PMF patients was 7.0-fold (95% CI = 1.41–33.1, p = 0.03) higher than in polycythemia vera patients. The inherited thrombophilia increased risk of thrombotic complication 5.4-fold (95% CI = 1.41–18.17, p = 0.01) in overall cohort of Ph-negative myeloproliferative neoplasms patients. This trend continued in Chernobyl patients (p = 0.02), but not in unexposed cases. Conclusions: Our findings confirm previous results of other studies reporting that the JAK2 V617F mutation significantly and independently influences on a disease phenotype in Ph-negative MPN. The inherited thrombophilia is important risk factors of the thrombosis development in overall cohort primary myelofibrosis patients, and especially in disease developed following radiation exposure. 2013 Article Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine / O.Y. Mishcheniuk, O.M. Kostukevich, I.V. Dmytrenko, V.V. Sholoyko, I.M. Prokopenko, Z.V. Martіna, G.V. Pilipenko, S.V. Klymenko // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 202-206. — Бібліогр.: 22 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145251 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Mishcheniuk, O.Y.
Kostukevich, O.M.
Dmytrenko, I.V.
Sholoyko, V.V.
Prokopenko, I.M.
Martіna, Z.V.
Pilipenko, G.V.
Klymenko, S.V.
Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
Experimental Oncology
description Aim: The aim of this study was to examine the JAK2 V617F, the G1691A allele of factor V, and the G20210A prothrombin gene mutation status, and their predictive value for thrombosis in patients with Ph-negative myeloproliferative neoplasms (MPN) in Ukraine, with special emphasize to patient exposed to ionizing radiation due to the Chernobyl accident. Materials and Methods: There were 198 patients with Ph-negative MPN included in the study. Of these, 45 patients had experienced radiation exposure due to the Chernobyl accident. The JAK2 V617F mutation, the G1691A of factor V and the G20210A of prothrombin were detected by allele-specific polymerase chain reaction. Results: The polycythemia vera and essential thrombocythemia patients in unexposed group and Chernobyl patients were comparable in terms of the JAK2 V617F mutation prevalence with the frequency of anomaly corresponding well to the published data on unselected cases of these types of Ph-negative MPN. The JAK2 V617F mutation was less common on the border of statistical significance (p = 0.08) in Chernobyl primary myelofibrosis (PMF) patients than in non-exposed patients. JAK2 V617F positive patients had higher level of leukocytes (p = 0.03), hemoglobin (p =0.04) and splenomegaly (p = 0.04) than those without mutation. The JAK2 V617F mutation was strong predictor for thrombosis in essential thrombocytemia patients (relative risk=3.1, 95% CI = 1.7–16.4, p = 0.03). In PMF, the association with thrombosis was found for the G1691A allele of factor V (p = 0.03). The risk of thrombosis associated with the inherited thrombophilia in PMF patients was 7.0-fold (95% CI = 1.41–33.1, p = 0.03) higher than in polycythemia vera patients. The inherited thrombophilia increased risk of thrombotic complication 5.4-fold (95% CI = 1.41–18.17, p = 0.01) in overall cohort of Ph-negative myeloproliferative neoplasms patients. This trend continued in Chernobyl patients (p = 0.02), but not in unexposed cases. Conclusions: Our findings confirm previous results of other studies reporting that the JAK2 V617F mutation significantly and independently influences on a disease phenotype in Ph-negative MPN. The inherited thrombophilia is important risk factors of the thrombosis development in overall cohort primary myelofibrosis patients, and especially in disease developed following radiation exposure.
format Article
author Mishcheniuk, O.Y.
Kostukevich, O.M.
Dmytrenko, I.V.
Sholoyko, V.V.
Prokopenko, I.M.
Martіna, Z.V.
Pilipenko, G.V.
Klymenko, S.V.
author_facet Mishcheniuk, O.Y.
Kostukevich, O.M.
Dmytrenko, I.V.
Sholoyko, V.V.
Prokopenko, I.M.
Martіna, Z.V.
Pilipenko, G.V.
Klymenko, S.V.
author_sort Mishcheniuk, O.Y.
title Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
title_short Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
title_full Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
title_fullStr Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
title_full_unstemmed Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine
title_sort molecular characterization of ph-negative myeloproliferative neoplasms in ukraine
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2013
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145251
citation_txt Molecular characterization of PH-negative myeloproliferative neoplasms in Ukraine / O.Y. Mishcheniuk, O.M. Kostukevich, I.V. Dmytrenko, V.V. Sholoyko, I.M. Prokopenko, Z.V. Martіna, G.V. Pilipenko, S.V. Klymenko // Experimental Oncology. — 2013. — Т. 35, № 3. — С. 202-206. — Бібліогр.: 22 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:21:39Z
last_indexed 2023-05-20T17:21:39Z
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