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Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells

Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells

Background and Aim: Human beta-defensin-2 (hBD-2) is an antimicrobial cationic peptide capable to control human carcinoma cell growth via cell cycle regulation. The present study was aimed on determination of hBD-2 influence on the growth patterns and malignant potential of cultured human melanoma c...

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Main Authors: Gerashchenko, O., Zhuravel, E., Skachkova, O., Khranovska, N., Pushkarev, V., Pogrebnoy, P., Soldatkina, M.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
Series:Experimental Oncology
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Original contributions
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/145313
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spelling irk-123456789-1453132019-01-21T01:23:42Z Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells Gerashchenko, O. Zhuravel, E. Skachkova, O. Khranovska, N. Pushkarev, V. Pogrebnoy, P. Soldatkina, M. Original contributions Background and Aim: Human beta-defensin-2 (hBD-2) is an antimicrobial cationic peptide capable to control human carcinoma cell growth via cell cycle regulation. The present study was aimed on determination of hBD-2 influence on the growth patterns and malignant potential of cultured human melanoma cells. Methods: The study was performed on cultured human melanoma cells of mel Z and mel Is lines treated with recombinant hBD-2 (rec-hBD-2); cell viability, proliferation, cell cycle distribution, and anchorage-independent growth were analyzed using MTT test, direct cell counting, flow cytometry, and colony forming assay respectively. Expression and/or phosphorylation levels of proteins involved in cell cycle control were evaluated by Western blotting. Results: The treatment of mel Z and mel Is cells with rec-hBD-2 in a concentration range of 100–1000 nM resulted in a concentration-dependent suppression of cell proliferation, viability, and colony forming activity. It has been shown that rec-hBD-2 exerts its growth suppression effects via significant downregulation of B-Raf expression, activation of pRB and upregulation of p21WAF1 expression, downregulation of cyclin D1 and cyclin E resulting in cell cycle arrest at G1/S checkpoint. Conclusion: According to obtained results, hBD-2 exerts its growth suppression effect toward human melanoma cells via downregulation of B-Raf, cyclin D1 and cyclin E expression, upregulation of p21WAF1 expression and activation of pRB. Key Words: human beta-defensin-2, melanoma, proliferation, viability, cell cycle, B-Raf, anchorage-independent growth. 2014 Article Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells / O. Gerashchenko, E. Zhuravel, O. Skachkova, N. Khranovska, V. Pushkarev, P. Pogrebnoy, M. Soldatkina // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 17-23. — Бібліогр.: 35 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145313 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Gerashchenko, O.
Zhuravel, E.
Skachkova, O.
Khranovska, N.
Pushkarev, V.
Pogrebnoy, P.
Soldatkina, M.
Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
Experimental Oncology
description Background and Aim: Human beta-defensin-2 (hBD-2) is an antimicrobial cationic peptide capable to control human carcinoma cell growth via cell cycle regulation. The present study was aimed on determination of hBD-2 influence on the growth patterns and malignant potential of cultured human melanoma cells. Methods: The study was performed on cultured human melanoma cells of mel Z and mel Is lines treated with recombinant hBD-2 (rec-hBD-2); cell viability, proliferation, cell cycle distribution, and anchorage-independent growth were analyzed using MTT test, direct cell counting, flow cytometry, and colony forming assay respectively. Expression and/or phosphorylation levels of proteins involved in cell cycle control were evaluated by Western blotting. Results: The treatment of mel Z and mel Is cells with rec-hBD-2 in a concentration range of 100–1000 nM resulted in a concentration-dependent suppression of cell proliferation, viability, and colony forming activity. It has been shown that rec-hBD-2 exerts its growth suppression effects via significant downregulation of B-Raf expression, activation of pRB and upregulation of p21WAF1 expression, downregulation of cyclin D1 and cyclin E resulting in cell cycle arrest at G1/S checkpoint. Conclusion: According to obtained results, hBD-2 exerts its growth suppression effect toward human melanoma cells via downregulation of B-Raf, cyclin D1 and cyclin E expression, upregulation of p21WAF1 expression and activation of pRB. Key Words: human beta-defensin-2, melanoma, proliferation, viability, cell cycle, B-Raf, anchorage-independent growth.
format Article
author Gerashchenko, O.
Zhuravel, E.
Skachkova, O.
Khranovska, N.
Pushkarev, V.
Pogrebnoy, P.
Soldatkina, M.
author_facet Gerashchenko, O.
Zhuravel, E.
Skachkova, O.
Khranovska, N.
Pushkarev, V.
Pogrebnoy, P.
Soldatkina, M.
author_sort Gerashchenko, O.
title Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
title_short Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
title_full Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
title_fullStr Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
title_full_unstemmed Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
title_sort involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2014
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145313
citation_txt Involvement of human beta-defensin-2 in regulation of malignant potential of cultured human melanoma cells / O. Gerashchenko, E. Zhuravel, O. Skachkova, N. Khranovska, V. Pushkarev, P. Pogrebnoy, M. Soldatkina // Experimental Oncology. — 2014. — Т. 36, № 1. — С. 17-23. — Бібліогр.: 35 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:21:48Z
last_indexed 2023-05-20T17:21:48Z
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