Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells
Aim: The aim of our study is to investigate the disorders of ferritin functioning in breast cancer (BC) cells of different molecular subtype. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A, and 184A1. Ferritin heavy chains (FTH) expres...
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| Дата: | 2014 |
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| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2014
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145363 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells / S.V. Chekhun, N.Y. Lukyanova, Y.V. Shvets, A.P. Burlaka, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 179-183. — Бібліогр.: 31 назв. — англ. |
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irk-123456789-1453632019-01-21T01:24:18Z Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells Chekhun, S.V. Lukyanova, N.Y. Shvets, Y.V. Burlaka, A.P. Buchynska, L.G. Original contributions Aim: The aim of our study is to investigate the disorders of ferritin functioning in breast cancer (BC) cells of different molecular subtype. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A, and 184A1. Ferritin heavy chains (FTH) expression was studied by immunohistochemical method. “Free iron” content and superoxide dismutase (SOD) activity were determined by means of EPR spectroscopy. Reactive oxygen species (ROS) level and peculiarities of microRNA expression in studied cell lines were evaluated using flow cytometry and PCR analysis, respectively. Results: It has been demonstrated that FTH expression directly correlates with proliferative activity of cells of both luminal (r = 0.51) and basal subtypes (r = 0.25), inversely correlates with expression of steroid hormones in cells of basal subtype (ER: r = −0.46; PR: r = −0.44) and does not depend on tumorigenic activity of both subtypes of studied cells (r = 0.12 and r = 0.9). Obtained data are the evidence that cells of luminal subtype B (MCF-7 cell line) and basal subtype (MDA-MB-231 and MDA-MB-468 cell lines) with high proliferative activity contain the highest level of free iron (2.9 ± 0.19·1016and 3.0 ± 0.22·1016) that can be consequence of intensive use of this element by cells, which actively divide and grow. Along with it, in cell of lines of basal subtype MDA-MB-231 and MDA-MB-468, high level of FTH (254 ± 2.3 and 270 ± 1.9) is being detected in consequence of increase of level of free iron, ROS (11.3 ± 1.05 and 7.27 ± 0.26) and SOD (9.4 ± 0.24 and 8.5 ± 0.18) as well as decrease of expression of microRNA 200b. In contrast, cells of luminal subtype B of MCF-7 line were distinguished by high expression of microRNA 200b and low ferritin level (125 ± 2.7). Conclusion: Obtained data demonstrate that tumor cells, which are referred to different molecular subtypes, are characterized by changes in system of support of balance of intercellular iron and certain associations of studied factors. Key Words: breast cancer, cell lines, luminal subtype, basal subtype, ferritin, “free iron”, ROS, SOD, miRNA 200b. 2014 Article Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells / S.V. Chekhun, N.Y. Lukyanova, Y.V. Shvets, A.P. Burlaka, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 179-183. — Бібліогр.: 31 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145363 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Chekhun, S.V. Lukyanova, N.Y. Shvets, Y.V. Burlaka, A.P. Buchynska, L.G. Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells Experimental Oncology |
| description |
Aim: The aim of our study is to investigate the disorders of ferritin functioning in breast cancer (BC) cells of different molecular subtype. Materials and Methods: The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A, and 184A1. Ferritin heavy chains (FTH) expression was studied by immunohistochemical method. “Free iron” content and superoxide dismutase (SOD) activity were determined by means of EPR spectroscopy. Reactive oxygen species (ROS) level and peculiarities of microRNA expression in studied cell lines were evaluated using flow cytometry and PCR analysis, respectively. Results: It has been demonstrated that FTH expression directly correlates with proliferative activity of cells of both luminal (r = 0.51) and basal subtypes (r = 0.25), inversely correlates with expression of steroid hormones in cells of basal subtype (ER: r = −0.46; PR: r = −0.44) and does not depend on tumorigenic activity of both subtypes of studied cells (r = 0.12 and r = 0.9). Obtained data are the evidence that cells of luminal subtype B (MCF-7 cell line) and basal subtype (MDA-MB-231 and MDA-MB-468 cell lines) with high proliferative activity contain the highest level of free iron (2.9 ± 0.19·1016and 3.0 ± 0.22·1016) that can be consequence of intensive use of this element by cells, which actively divide and grow. Along with it, in cell of lines of basal subtype MDA-MB-231 and MDA-MB-468, high level of FTH (254 ± 2.3 and 270 ± 1.9) is being detected in consequence of increase of level of free iron, ROS (11.3 ± 1.05 and 7.27 ± 0.26) and SOD (9.4 ± 0.24 and 8.5 ± 0.18) as well as decrease of expression of microRNA 200b. In contrast, cells of luminal subtype B of MCF-7 line were distinguished by high expression of microRNA 200b and low ferritin level (125 ± 2.7). Conclusion: Obtained data demonstrate that tumor cells, which are referred to different molecular subtypes, are characterized by changes in system of support of balance of intercellular iron and certain associations of studied factors. Key Words: breast cancer, cell lines, luminal subtype, basal subtype, ferritin, “free iron”, ROS, SOD, miRNA 200b. |
| format |
Article |
| author |
Chekhun, S.V. Lukyanova, N.Y. Shvets, Y.V. Burlaka, A.P. Buchynska, L.G. |
| author_facet |
Chekhun, S.V. Lukyanova, N.Y. Shvets, Y.V. Burlaka, A.P. Buchynska, L.G. |
| author_sort |
Chekhun, S.V. |
| title |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| title_short |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| title_full |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| title_fullStr |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| title_full_unstemmed |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| title_sort |
significance of ferritin expression in formation of malignant phenotype of human breast cancer cells |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2014 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145363 |
| citation_txt |
Significance of ferritin expression in formation of malignant phenotype of human breast cancer cells / S.V. Chekhun, N.Y. Lukyanova, Y.V. Shvets, A.P. Burlaka, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 179-183. — Бібліогр.: 31 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-05-20T17:21:57Z |
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2023-05-20T17:21:57Z |
| _version_ |
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