2025-02-23T06:19:44-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: Query fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-145365%22&qt=morelikethis&rows=5
2025-02-23T06:19:44-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: => GET http://localhost:8983/solr/biblio/select?fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-145365%22&qt=morelikethis&rows=5
2025-02-23T06:19:44-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: <= 200 OK
2025-02-23T06:19:44-05:00 DEBUG: Deserialized SOLR response
The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer

The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer

Aim: The aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats. Materials and Methods: Gastric carcinoge­nesis was initiated in 80 white male rats by 10...

Full description

Saved in:
Bibliographic Details
Main Authors: Tymoshenko, M.O., Kravchenko, O.O., Sokur, O.V., Ostapchenko, L.I.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
Series:Experimental Oncology
Subjects:
Original contributions
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/145365
Tags: Add Tag
No Tags, Be the first to tag this record!
id irk-123456789-145365
record_format dspace
spelling irk-123456789-1453652019-01-21T01:23:04Z The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer Tymoshenko, M.O. Kravchenko, O.O. Sokur, O.V. Ostapchenko, L.I. Original contributions Aim: The aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats. Materials and Methods: Gastric carcinoge­nesis was initiated in 80 white male rats by 10-week replacement of drinking water with 0.01% solution of N-methyl-N´-nitro-N-nitrosoguanidine, at the same time they were redefined on diet containing 5% NaCl. Then the animals were fed with standard vivarium diet till the end of 24th week. The gastric mucosa cells were examined at the end of 4th, 6th, 8th, 10th, 12th, 18th, and 24th weeks. Sandwich ELISA method was used to determine the content of phospho-c-Jun. The contents of GSH and GSSG were analyzed by spectrofluorymetric method with the use of orthophthalic aldehyde. Results: At the end of 6th, 8th, 10th weeks of MNNG and NaCl treatment the gastric mucosa cells were characterized by 4-, 6.3-, 1.9-fold higher content of phospho-c-Jun compared to the control, respectively, and 12, 18 and 24 weeks there was registered a stable increase of phospho-c-Jun content on the average at 3.6-fold compared to control values. Starting from 6th week of gastric cancer development an average decrease of GSH/GSSG was 3.4-fold compared with the control. Conclusion: During gastric carcinogenesis there was registered the decrease of GSH/GSSG ratio and increased level of phosphorylated c-Jun what points on MAP-kinase cascade activation in prooxidant conditions. Key Words: c-Jun, reduce glutathione (GSH), oxidized glutathione (GSSG), gastric cancer. 2014 Article The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer / M.O. Tymoshenko, O.O. Kravchenko, O.V. Sokur, L.I. Ostapchenko // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 191-195. — Бібліогр.: 27 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145365 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Tymoshenko, M.O.
Kravchenko, O.O.
Sokur, O.V.
Ostapchenko, L.I.
The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
Experimental Oncology
description Aim: The aim of the study was to determine the phosphorylated c-Jun content and reduced and oxidized glutathione (GSH/GSSG) ratio in gastric mucosa cells during the process of gastric cancer development in rats. Materials and Methods: Gastric carcinoge­nesis was initiated in 80 white male rats by 10-week replacement of drinking water with 0.01% solution of N-methyl-N´-nitro-N-nitrosoguanidine, at the same time they were redefined on diet containing 5% NaCl. Then the animals were fed with standard vivarium diet till the end of 24th week. The gastric mucosa cells were examined at the end of 4th, 6th, 8th, 10th, 12th, 18th, and 24th weeks. Sandwich ELISA method was used to determine the content of phospho-c-Jun. The contents of GSH and GSSG were analyzed by spectrofluorymetric method with the use of orthophthalic aldehyde. Results: At the end of 6th, 8th, 10th weeks of MNNG and NaCl treatment the gastric mucosa cells were characterized by 4-, 6.3-, 1.9-fold higher content of phospho-c-Jun compared to the control, respectively, and 12, 18 and 24 weeks there was registered a stable increase of phospho-c-Jun content on the average at 3.6-fold compared to control values. Starting from 6th week of gastric cancer development an average decrease of GSH/GSSG was 3.4-fold compared with the control. Conclusion: During gastric carcinogenesis there was registered the decrease of GSH/GSSG ratio and increased level of phosphorylated c-Jun what points on MAP-kinase cascade activation in prooxidant conditions. Key Words: c-Jun, reduce glutathione (GSH), oxidized glutathione (GSSG), gastric cancer.
format Article
author Tymoshenko, M.O.
Kravchenko, O.O.
Sokur, O.V.
Ostapchenko, L.I.
author_facet Tymoshenko, M.O.
Kravchenko, O.O.
Sokur, O.V.
Ostapchenko, L.I.
author_sort Tymoshenko, M.O.
title The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
title_short The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
title_full The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
title_fullStr The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
title_full_unstemmed The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer
title_sort phospho-c-jun (ser63) content in mucosa cells under experimental development of gastric cancer
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2014
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145365
citation_txt The phospho-c-JUN (Ser63) content in mucosa cells under experimental development of gastric cancer / M.O. Tymoshenko, O.O. Kravchenko, O.V. Sokur, L.I. Ostapchenko // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 191-195. — Бібліогр.: 27 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT tymoshenkomo thephosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT kravchenkooo thephosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT sokurov thephosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT ostapchenkoli thephosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT tymoshenkomo phosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT kravchenkooo phosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT sokurov phosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
AT ostapchenkoli phosphocjunser63contentinmucosacellsunderexperimentaldevelopmentofgastriccancer
first_indexed 2023-05-20T17:21:57Z
last_indexed 2023-05-20T17:21:57Z
_version_ 1796153130760536064

Similar Items