Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin

Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin

Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of...

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Бібліографічні деталі
Дата:2014
Автори: Chekhun, V.F., Lozovska, Y.V., Burlaka, A.P., Lukyanova, N.Y., Todor, I.N., Naleskina, L.A.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145366
Теги: Додати тег
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
id irk-123456789-145366
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spelling irk-123456789-1453662019-01-22T01:23:43Z Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin Chekhun, V.F. Lozovska, Y.V. Burlaka, A.P. Lukyanova, N.Y. Todor, I.N. Naleskina, L.A. Original contributions Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating acti­vity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectro­scopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils. 2014 Article Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145366 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
Experimental Oncology
description Aim : To study in vivo the peculiarities of changes of iron metabolism and antioxidant system in dynamics of growth of Guerin carcinoma with different sensitivity to cisplatin. Materials and Methods: In order to evaluate the content of metallothionein-1 (MT-1) in tumor homogenates and blood serum of rats with cisplatin-sensitive and cisplatin-resistant Guerin carcinoma the immunoenzyme method was used. The evaluation of ceruloplasmin activity, content of “free iron” complexes, superoxide and NO-generating acti­vity of NADPH-oxidase and iNOS activity in neutrophils, blood serum and tumor homogenates was measured by EPR-spectro­scopy. Results: Maximal accumulation of MT-1 in blood serum and tumor, more pronounced in resistant strain, at the border of latent and exponential phase of growth has been shown that is the evidence of protective role of this protein in the respect to the generation of free radical compounds. It has been determined that in animals with cisplatin-resistant strain of Guerin carcinoma, increase of “free iron” complexes is more apparent both on the level of tumor and organism on the background on increase of CP/TR ratio that is the consequence of organism antioxidant protection system disorder. Conclutions: Mentioned changes in metabolism of iron with its accumulation in tumor and further reprogramming of mitochondria metabolism and activity of NADPH-oxidase for non-transformed cells are favorable conditions for the formation of oxidative phenotype of tumor. Key Words: Guerin carcinoma, metallothionein-1, antioxidant system, transferrin, ceruloplasmin, “free iron” complexes, activity of NADPH-oxidase and iNOS neutrophils.
format Article
author Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
author_facet Chekhun, V.F.
Lozovska, Y.V.
Burlaka, A.P.
Lukyanova, N.Y.
Todor, I.N.
Naleskina, L.A.
author_sort Chekhun, V.F.
title Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_short Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_full Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_fullStr Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_full_unstemmed Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
title_sort peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2014
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145366
citation_txt Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin / V.F. Chekhun, Y.V. Lozovska, A.P. Burlaka, N.Y. Lukyanova, I.N. Todor, L.A. Naleskina // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 196-201. — Бібліогр.: 26 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:21:58Z
last_indexed 2023-05-20T17:21:58Z
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