The study of chromosomal instability in patients with endometrial cancer
Aim: Study is devoted to evaluation of sensitivity of peripheral blood T-lymphocytes (PBL) of patients with endometrial cancer (EC) to genotoxic effect of bleomycin and detection of patients with hidden chromosomal instability. Methods: PBL of 24 EC patients (mean age 58.9 ± 2.9) and 10 healthy wome...
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| Дата: | 2014 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2014
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145367 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The study of chromosomal instability in patients with endometrial cancer / I.P. Nesina, N.P. Iurchenko, S.V. Nespryad’ko, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 202-206. — Бібліогр.: 24 назв. — англ. |
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irk-123456789-1453672019-01-22T01:23:16Z The study of chromosomal instability in patients with endometrial cancer Nesina, I.P. Lurchenko, N.P. Nespryadko, S.V. Buchynska, L.G. Original contributions Aim: Study is devoted to evaluation of sensitivity of peripheral blood T-lymphocytes (PBL) of patients with endometrial cancer (EC) to genotoxic effect of bleomycin and detection of patients with hidden chromosomal instability. Methods: PBL of 24 EC patients (mean age 58.9 ± 2.9) and 10 healthy women-volunteers (mean age 55.7 ± 2.3) were subjected to cytogenetic analysis. Results: Mean spontaneous level of chromosomal aberrations (CA) per 100 analyzed lymphocytes (CA/100) of healthy women has equaled 2.7 ± 0.6, i.e. has not exceeded maximal values of healthy population and was significantly lower (p < 0.05), than in PBL of EC patients (6.9 ± 0.6). After incubation of PBL with bleomycin, number of CA/100 significantly was increased both in control (11.5 ± 1.3) and in EC patients (21.9 ± 1.0). Spontaneous chromosomal instability has been observed in 41.7%, increased sensitivity to bleomycin — in 54.2% and hidden chromosomal instability in 37.5% of patients with EC. It has been shown that level of specific damage of genome in EC patients has constituted 2·10–5, and after exposure with bleomycin, it was increased 4.5 times (9·10–5) that was significantly higher (p < 0.05) compared to control (8·10–6 and 1.0·10–5, respectively). Conclusion: These results have demonstrated that PBL of most patients with EC are characterized by apparent genome alterations, which are manifested by increased number of spontaneous and induced chromosomal damages, hypersensitivity to mutagens and hidden chromosomal instability. Key Words: endometrial cancer, peripheral blood lymphocytes, chromosomal aberrations, hidden chromosomal instability. 2014 Article The study of chromosomal instability in patients with endometrial cancer / I.P. Nesina, N.P. Iurchenko, S.V. Nespryad’ko, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 202-206. — Бібліогр.: 24 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145367 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Nesina, I.P. Lurchenko, N.P. Nespryadko, S.V. Buchynska, L.G. The study of chromosomal instability in patients with endometrial cancer Experimental Oncology |
| description |
Aim: Study is devoted to evaluation of sensitivity of peripheral blood T-lymphocytes (PBL) of patients with endometrial cancer (EC) to genotoxic effect of bleomycin and detection of patients with hidden chromosomal instability. Methods: PBL of 24 EC patients (mean age 58.9 ± 2.9) and 10 healthy women-volunteers (mean age 55.7 ± 2.3) were subjected to cytogenetic analysis. Results: Mean spontaneous level of chromosomal aberrations (CA) per 100 analyzed lymphocytes (CA/100) of healthy women has equaled 2.7 ± 0.6, i.e. has not exceeded maximal values of healthy population and was significantly lower (p < 0.05), than in PBL of EC patients (6.9 ± 0.6). After incubation of PBL with bleomycin, number of CA/100 significantly was increased both in control (11.5 ± 1.3) and in EC patients (21.9 ± 1.0). Spontaneous chromosomal instability has been observed in 41.7%, increased sensitivity to bleomycin — in 54.2% and hidden chromosomal instability in 37.5% of patients with EC. It has been shown that level of specific damage of genome in EC patients has constituted 2·10–5, and after exposure with bleomycin, it was increased 4.5 times (9·10–5) that was significantly higher (p < 0.05) compared to control (8·10–6 and 1.0·10–5, respectively). Conclusion: These results have demonstrated that PBL of most patients with EC are characterized by apparent genome alterations, which are manifested by increased number of spontaneous and induced chromosomal damages, hypersensitivity to mutagens and hidden chromosomal instability. Key Words: endometrial cancer, peripheral blood lymphocytes, chromosomal aberrations, hidden chromosomal instability. |
| format |
Article |
| author |
Nesina, I.P. Lurchenko, N.P. Nespryadko, S.V. Buchynska, L.G. |
| author_facet |
Nesina, I.P. Lurchenko, N.P. Nespryadko, S.V. Buchynska, L.G. |
| author_sort |
Nesina, I.P. |
| title |
The study of chromosomal instability in patients with endometrial cancer |
| title_short |
The study of chromosomal instability in patients with endometrial cancer |
| title_full |
The study of chromosomal instability in patients with endometrial cancer |
| title_fullStr |
The study of chromosomal instability in patients with endometrial cancer |
| title_full_unstemmed |
The study of chromosomal instability in patients with endometrial cancer |
| title_sort |
study of chromosomal instability in patients with endometrial cancer |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2014 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145367 |
| citation_txt |
The study of chromosomal instability in patients with endometrial cancer / I.P. Nesina, N.P. Iurchenko, S.V. Nespryad’ko, L.G. Buchynska // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 202-206. — Бібліогр.: 24 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-05-20T17:21:58Z |
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2023-05-20T17:21:58Z |
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