In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture

Aim: Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metasta...

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Дата:2014
Автори: Roomi, M.W., Bhanap, B., Roomi, N.W., Niedzwiecki, A., Rath, M.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2014
Назва видання:Experimental Oncology
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Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145369
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture / M.W. Roomi, B. Bhanap, N.W. Roomi, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 212-214. — Бібліогр.: 13 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1453692019-01-22T01:23:20Z In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture Roomi, M.W. Bhanap, B. Roomi, N.W. Niedzwiecki, A. Rath, M. Short communications Aim: Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metastasis. Biological agents that prevent extracellular matrix digestion by the MMPs have been shown to be promising therapeutic approaches to cancer. In this study, we investigated effects of a nutrient mixture (NM) containing, ascorbic acid, lysine, proline and green tea extract, on human FANCA and FANCC lymphoblasts for viability, MMP secretion and invasion. Methods: Human FANCA lymphoblasts GM13022 and HCS536 were challenged with NM at concentration range within 10–1000 µg/ml. Cell toxicity was assessed by Trypan blue dye exclusion test. Invasion was evaluated through Matrigel and gelatinase zymography for MMP activity. Results: NM was toxic in dose dependent mode to HCS536 cells but not to GM13022 cells. GM13022 cells but not HCS536 cells exhibited MMP-9 secretion, which was inhibited by NM. Matrigel invasion was inhibited in HCS536 cells at 100 and 500 µg/ml by 27% and 93%, respectively. In GM13022 cells, the NM showed completely blocked Matrigel invasion at 500 µg/ml. Conclusion: NM inhibited MMP secretion and Matrigel invasion in FANCA and inhibited invasion and induced toxicity in FANCC lymphoblasts. These results suggest that the NM may have therapeutic potential in Fanconi anemia associated neoplasia. Key Words: Fanconi anemia, nutrient mixture, MMP, Matrigel invasion. 2014 Article In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture / M.W. Roomi, B. Bhanap, N.W. Roomi, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 212-214. — Бібліогр.: 13 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145369 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Short communications
Short communications
spellingShingle Short communications
Short communications
Roomi, M.W.
Bhanap, B.
Roomi, N.W.
Niedzwiecki, A.
Rath, M.
In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
Experimental Oncology
description Aim: Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metastasis. Biological agents that prevent extracellular matrix digestion by the MMPs have been shown to be promising therapeutic approaches to cancer. In this study, we investigated effects of a nutrient mixture (NM) containing, ascorbic acid, lysine, proline and green tea extract, on human FANCA and FANCC lymphoblasts for viability, MMP secretion and invasion. Methods: Human FANCA lymphoblasts GM13022 and HCS536 were challenged with NM at concentration range within 10–1000 µg/ml. Cell toxicity was assessed by Trypan blue dye exclusion test. Invasion was evaluated through Matrigel and gelatinase zymography for MMP activity. Results: NM was toxic in dose dependent mode to HCS536 cells but not to GM13022 cells. GM13022 cells but not HCS536 cells exhibited MMP-9 secretion, which was inhibited by NM. Matrigel invasion was inhibited in HCS536 cells at 100 and 500 µg/ml by 27% and 93%, respectively. In GM13022 cells, the NM showed completely blocked Matrigel invasion at 500 µg/ml. Conclusion: NM inhibited MMP secretion and Matrigel invasion in FANCA and inhibited invasion and induced toxicity in FANCC lymphoblasts. These results suggest that the NM may have therapeutic potential in Fanconi anemia associated neoplasia. Key Words: Fanconi anemia, nutrient mixture, MMP, Matrigel invasion.
format Article
author Roomi, M.W.
Bhanap, B.
Roomi, N.W.
Niedzwiecki, A.
Rath, M.
author_facet Roomi, M.W.
Bhanap, B.
Roomi, N.W.
Niedzwiecki, A.
Rath, M.
author_sort Roomi, M.W.
title In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
title_short In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
title_full In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
title_fullStr In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
title_full_unstemmed In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture
title_sort in vitro inhibition of matrix metalloproteinases, invasion and growth of fanconi anemia human fanca and fancc lymphoblasts by nutrient mixture
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2014
topic_facet Short communications
url http://dspace.nbuv.gov.ua/handle/123456789/145369
citation_txt In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture / M.W. Roomi, B. Bhanap, N.W. Roomi, A. Niedzwiecki, M. Rath // Experimental Oncology. — 2014. — Т. 36, № 3. — С. 212-214. — Бібліогр.: 13 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:21:58Z
last_indexed 2023-05-20T17:21:58Z
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