Prognostic significance of MDM2 gene expression in childhood neuroblastoma

Prognostic significance of MDM2 gene expression in childhood neuroblastoma

Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissu...

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Бібліографічні деталі
Дата:2015
Автори: Inomistova, M.V., Svergun, N.M., Khranovska, N.M., Skachkova, O.V., Gorbach, O.I., Klymnyuk, G.I.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145465
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1454652019-01-23T01:23:46Z Prognostic significance of MDM2 gene expression in childhood neuroblastoma Inomistova, M.V. Svergun, N.M. Khranovska, N.M. Skachkova, O.V. Gorbach, O.I. Klymnyuk, G.I. Original contributions Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissue samples of total 91 neuroblastoma patients (mean age: 39.45 ± 4.81 months). MDM2 mRNA levels were detected with Q-PCR. TP53 gene deletion was detected with FISH method. MYCN amplification was detected with ­Q-PCR analysis in fresh tumor samples and FISH in FFPE samples. Results: We investigated the association of MDM2 mRNA expression with clinical outcome in neuroblastoma patients (n = 91). Kaplan — Meier curves showed a significant association of high MDM2 expression with poor event-free survival (p < 0.001). Clinical outcome of patients without MYCN amplification with low MDM2 expression was associated with better event-free survival than with high MDM2 expression (p < 0.001). Overexpression of MDM2 can be used as significant prognostic marker for patient stratification on risk groups and treatment optimization. Conclusion: Our results showed that the high expression of MDM2 at mRNA levels is an important factor of neuroblastoma prognosis. It may be a valuable additional molecular marker in guiding specific therapy in MYCN non-amplified NB patients without TP53 gene deletion. Key Words: neuroblastoma, MDM2 mRNA expression, MYCN gene amplification, p53/MDM2 interaction. 2015 Article Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145465 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Inomistova, M.V.
Svergun, N.M.
Khranovska, N.M.
Skachkova, O.V.
Gorbach, O.I.
Klymnyuk, G.I.
Prognostic significance of MDM2 gene expression in childhood neuroblastoma
Experimental Oncology
description Aim: To investigate the association of MDM2 expression at the mRNA levels in neuroblastoma with clinical features and unfavorable disease factors to determine the possibility of it usage as a prognostic marker of neuroblastoma. Materials and Methods: Total RNA and DNA were extracted from tumor tissue samples of total 91 neuroblastoma patients (mean age: 39.45 ± 4.81 months). MDM2 mRNA levels were detected with Q-PCR. TP53 gene deletion was detected with FISH method. MYCN amplification was detected with ­Q-PCR analysis in fresh tumor samples and FISH in FFPE samples. Results: We investigated the association of MDM2 mRNA expression with clinical outcome in neuroblastoma patients (n = 91). Kaplan — Meier curves showed a significant association of high MDM2 expression with poor event-free survival (p < 0.001). Clinical outcome of patients without MYCN amplification with low MDM2 expression was associated with better event-free survival than with high MDM2 expression (p < 0.001). Overexpression of MDM2 can be used as significant prognostic marker for patient stratification on risk groups and treatment optimization. Conclusion: Our results showed that the high expression of MDM2 at mRNA levels is an important factor of neuroblastoma prognosis. It may be a valuable additional molecular marker in guiding specific therapy in MYCN non-amplified NB patients without TP53 gene deletion. Key Words: neuroblastoma, MDM2 mRNA expression, MYCN gene amplification, p53/MDM2 interaction.
format Article
author Inomistova, M.V.
Svergun, N.M.
Khranovska, N.M.
Skachkova, O.V.
Gorbach, O.I.
Klymnyuk, G.I.
author_facet Inomistova, M.V.
Svergun, N.M.
Khranovska, N.M.
Skachkova, O.V.
Gorbach, O.I.
Klymnyuk, G.I.
author_sort Inomistova, M.V.
title Prognostic significance of MDM2 gene expression in childhood neuroblastoma
title_short Prognostic significance of MDM2 gene expression in childhood neuroblastoma
title_full Prognostic significance of MDM2 gene expression in childhood neuroblastoma
title_fullStr Prognostic significance of MDM2 gene expression in childhood neuroblastoma
title_full_unstemmed Prognostic significance of MDM2 gene expression in childhood neuroblastoma
title_sort prognostic significance of mdm2 gene expression in childhood neuroblastoma
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145465
citation_txt Prognostic significance of MDM2 gene expression in childhood neuroblastoma / M.V. Inomistova, N.M. Svergun, N.M. Khranovska, O.V. Skachkova, O.I. Gorbach, G.I. Klymnyuk // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 111-115. — Бібліогр.: 15 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:22:13Z
last_indexed 2023-05-20T17:22:13Z
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