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Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro

Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro

Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vul...

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Main Authors: Kochubei, T.O., Maksymchuk, O.V., Piven, O.O., Lukash, L.L.
Format: Article
Language:English
Published: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Series:Experimental Oncology
Subjects:
Original contributions
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/145466
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spelling irk-123456789-1454662019-01-23T01:23:05Z Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. Original contributions Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax. 2015 Article Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145466 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Kochubei, T.O.
Maksymchuk, O.V.
Piven, O.O.
Lukash, L.L.
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
Experimental Oncology
description Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax.
format Article
author Kochubei, T.O.
Maksymchuk, O.V.
Piven, O.O.
Lukash, L.L.
author_facet Kochubei, T.O.
Maksymchuk, O.V.
Piven, O.O.
Lukash, L.L.
author_sort Kochubei, T.O.
title Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
title_short Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
title_full Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
title_fullStr Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
title_full_unstemmed Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
title_sort isolectins of phytohemagglutinin are able to induce apoptosis in hep-2 carcinoma cells in vitro
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145466
citation_txt Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ.
series Experimental Oncology
work_keys_str_mv AT kochubeito isolectinsofphytohemagglutininareabletoinduceapoptosisinhep2carcinomacellsinvitro
AT maksymchukov isolectinsofphytohemagglutininareabletoinduceapoptosisinhep2carcinomacellsinvitro
AT pivenoo isolectinsofphytohemagglutininareabletoinduceapoptosisinhep2carcinomacellsinvitro
AT lukashll isolectinsofphytohemagglutininareabletoinduceapoptosisinhep2carcinomacellsinvitro
first_indexed 2023-05-20T17:22:13Z
last_indexed 2023-05-20T17:22:13Z
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