Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro
Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vul...
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| Дата: | 2015 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145466 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. |
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irk-123456789-1454662019-01-23T01:23:05Z Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. Original contributions Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax. 2015 Article Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145466 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro Experimental Oncology |
| description |
Aim: To study the effects of total phytohemagglutinin (PHA) and its isolectins on cell death and apoptosis in human HEp-2 carcinoma cells and to analyze the possible molecular mechanisms of lectin induced apoptosis. Materials and Methods: The commercial preparation of the kidney beans (Phaseolus vulgaris) lectins and HEp-2 cells were used. Apoptosis index was determined using acridine orange and ethidium bromide staining. The expression levels of apoptosis mediator cleaved caspase-3 and proapoptotic Bax protein were studied by Western blot analysis. The gene expression levels were analyzed by qPCR. Results: PHA and its isolectins induced apoptosis in HEp-2 cells accompanied by the increased expression of caspase-3 cleaved form, with PHA-E being the most effective. The treatment of HEp-2 cells with PHA or its isolectins resulted in a marked increase of Bax on both mRNA and protein levels. Conclusions: PHA and its isolectins were shown to induce the apoptosis in human HEp-2 carcinoma cells via increasing proapoptotic protein Bax and activating caspases-3. Key Words: phytohemagglutinin, isolectins, apoptosis, caspase-3, Bax. |
| format |
Article |
| author |
Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. |
| author_facet |
Kochubei, T.O. Maksymchuk, O.V. Piven, O.O. Lukash, L.L. |
| author_sort |
Kochubei, T.O. |
| title |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_short |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_full |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_fullStr |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_full_unstemmed |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro |
| title_sort |
isolectins of phytohemagglutinin are able to induce apoptosis in hep-2 carcinoma cells in vitro |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2015 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145466 |
| citation_txt |
Isolectins of phytohemagglutinin are able to induce apoptosis in HEp-2 carcinoma cells in vitro / T.O. Kochubei, O.V. Maksymchuk, O.O. Piven, L.L. Lukash // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 116-119. — Бібліогр.: 15 назв. — англ. |
| series |
Experimental Oncology |
| work_keys_str_mv |
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| first_indexed |
2023-05-20T17:22:13Z |
| last_indexed |
2023-05-20T17:22:13Z |
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