Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16

Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16

Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular an...

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Бібліографічні деталі
Дата:2015
Автори: Fedosova, N.I., Voeykova, I.M., Karaman, O.M., Symchych, T.V., Didenko, G.V., Lisovenko, G.S., Evstratieva, L.M., Potebnya, G.P.
Формат: Стаття
Мова:English
Опубліковано: Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України 2015
Назва видання:Experimental Oncology
Теми:
Original contributions
Онлайн доступ:http://dspace.nbuv.gov.ua/handle/123456789/145475
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Назва журналу:Digital Library of Periodicals of National Academy of Sciences of Ukraine
Цитувати:Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ.

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Digital Library of Periodicals of National Academy of Sciences of Ukraine
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spelling irk-123456789-1454752019-01-23T01:23:04Z Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 Fedosova, N.I. Voeykova, I.M. Karaman, O.M. Symchych, T.V. Didenko, G.V. Lisovenko, G.S. Evstratieva, L.M. Potebnya, G.P. Original contributions Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular and humoral chains of immune system, and to analyze possible mechanisms of its antimetastatic action. Materials and Methods: XCV was administered triply with 3-day intervals after surgical removal of experimental melanoma В-16 in C57Bl/6 mice. Cytotoxic activity (CTA) of splenocytes against target cells К-562 as well as CTA of splenocytes, peritoneal macrophages (PM) and blood serum against melanoma В-16 target cells were determined using МТТ test. The content of circulating immune complexes (CIC) in blood serum was evaluated by precipitation reaction. Results: Immunologic effects of XCV vaccination in experimental animals with surgically removed melanoma B-16 in comparison with similarly treated unvaccinated mice were as follows: prevention of medium molecular weight CIC accumulation in blood serum during all observation period, significant increase (р < 0.05) of CTA of effectors of unspecific antitumor immunity (natural killer cells — NK — by 25.5 ± 1.7 vs 12.5 ± 5.4%, and PM — by 37.3 ± 0.6 vs 32.0 ± 0.9%, respectively) at 37th day after the surgery, and also preservation of functional activity of specific cytotoxic lymphocytes at the level of intact control. Conclusion: The results of the study allow propose that antimetastatic effect of XCV vaccination could be based on increased CTA of NK and PM, and preservation of CTL functional activity at late terms after surgical removal of B-16 primary tumors. Key Words: xenogeneic cancer vaccine, melanoma В-16, natural killer cells, macrophages, cytotoxic lymphocytes, cytotoxic activity, antimetastatic activity. 2015 Article Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145475 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
institution Digital Library of Periodicals of National Academy of Sciences of Ukraine
collection DSpace DC
language English
topic Original contributions
Original contributions
spellingShingle Original contributions
Original contributions
Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
Experimental Oncology
description Aim: To study the effects of xenogeneic cancer vaccine (XCV) developed on the basis of nervous tissue antigen from rat embryo of late gestation period and protein-containing metabolite of Bacillus subtilis with molecular weight of 70 kDa, on specific and unspecific antitumor reactions of cellular and humoral chains of immune system, and to analyze possible mechanisms of its antimetastatic action. Materials and Methods: XCV was administered triply with 3-day intervals after surgical removal of experimental melanoma В-16 in C57Bl/6 mice. Cytotoxic activity (CTA) of splenocytes against target cells К-562 as well as CTA of splenocytes, peritoneal macrophages (PM) and blood serum against melanoma В-16 target cells were determined using МТТ test. The content of circulating immune complexes (CIC) in blood serum was evaluated by precipitation reaction. Results: Immunologic effects of XCV vaccination in experimental animals with surgically removed melanoma B-16 in comparison with similarly treated unvaccinated mice were as follows: prevention of medium molecular weight CIC accumulation in blood serum during all observation period, significant increase (р < 0.05) of CTA of effectors of unspecific antitumor immunity (natural killer cells — NK — by 25.5 ± 1.7 vs 12.5 ± 5.4%, and PM — by 37.3 ± 0.6 vs 32.0 ± 0.9%, respectively) at 37th day after the surgery, and also preservation of functional activity of specific cytotoxic lymphocytes at the level of intact control. Conclusion: The results of the study allow propose that antimetastatic effect of XCV vaccination could be based on increased CTA of NK and PM, and preservation of CTL functional activity at late terms after surgical removal of B-16 primary tumors. Key Words: xenogeneic cancer vaccine, melanoma В-16, natural killer cells, macrophages, cytotoxic lymphocytes, cytotoxic activity, antimetastatic activity.
format Article
author Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
author_facet Fedosova, N.I.
Voeykova, I.M.
Karaman, O.M.
Symchych, T.V.
Didenko, G.V.
Lisovenko, G.S.
Evstratieva, L.M.
Potebnya, G.P.
author_sort Fedosova, N.I.
title Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_short Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_full Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_fullStr Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_full_unstemmed Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16
title_sort cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma b-16
publisher Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
publishDate 2015
topic_facet Original contributions
url http://dspace.nbuv.gov.ua/handle/123456789/145475
citation_txt Cytotoxic activity of immune cells following administration of xenogeneic cancer vaccine in mice with melanoma B-16 / N.I. Fedosova, I.M. Voeykova, O.M. Karaman, T.V. Symchych, G.V. Didenko, G.S. Lisovenko, L.M. Evstratieva, G.P. Potebnya // Experimental Oncology. — 2015. — Т. 37, № 2. — С. 130-134. — Бібліогр.: 38 назв. — англ.
series Experimental Oncology
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first_indexed 2023-05-20T17:22:15Z
last_indexed 2023-05-20T17:22:15Z
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