Application of serex-analysis for identification of human colon cancer antigens
Background: Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera i...
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| Дата: | 2015 |
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| Автори: | , , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2015
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145484 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Application of serex-analysis for identification of human colon cancer antigens / O.M. Garifulin, V.O. Kykot, N.Y. Gridina, R.G. Kiyamova, I.T. Gout, V.V. Filonenko // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 173-180. — Бібліогр.: 46 назв. — англ. |
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irk-123456789-1454842019-01-23T01:23:55Z Application of serex-analysis for identification of human colon cancer antigens Garifulin, O.M. Kykot, V.O. Gridina, N.Y. Kiyamova, R.G. Gout, I.T. Filonenko, V.V. Original contributions Background: Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera is more productive for antigen isolation, SEREX-analysis was applied to four cases of stages II–IV primary colon tumor and 22 new antigens were isolated. Objective: To characterize 22 primary colon cancer antigens isolated by SEREX-technique. Materials and Methods: Allogenic screening, real-time PCR analysis. Results: After allogeneic immunoscreening, for 5 of 22 (22%) isolated antigens were confirmed colon cancer restricted serological profile solely positive for 14% of tested colon cancer sera. Through these five antigens, KY-CC-17/β-actin has cytoskeleton function; KY-CC-14/ACTR1A and KY-CC-19/TSGA2 participate in chromosome segregation; KY-CC-12/FKBP4 regulates steroid receptor function and KY-CC-15/PLRG1 is a component of spliceosome complex. For the last four antigens tested were found aberrant mRNA expression in some cases of colon tumor. Conclusion: The exploration of identified antigens may define suitable targets for immunotherapy or diagnostic of colon cancer. Key Words: colon cancer, SEREX-analysis, allogeneic immunoscreening. 2015 Article Application of serex-analysis for identification of human colon cancer antigens / O.M. Garifulin, V.O. Kykot, N.Y. Gridina, R.G. Kiyamova, I.T. Gout, V.V. Filonenko // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 173-180. — Бібліогр.: 46 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145484 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
| spellingShingle |
Original contributions Original contributions Garifulin, O.M. Kykot, V.O. Gridina, N.Y. Kiyamova, R.G. Gout, I.T. Filonenko, V.V. Application of serex-analysis for identification of human colon cancer antigens Experimental Oncology |
| description |
Background: Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera is more productive for antigen isolation, SEREX-analysis was applied to four cases of stages II–IV primary colon tumor and 22 new antigens were isolated. Objective: To characterize 22 primary colon cancer antigens isolated by SEREX-technique. Materials and Methods: Allogenic screening, real-time PCR analysis. Results: After allogeneic immunoscreening, for 5 of 22 (22%) isolated antigens were confirmed colon cancer restricted serological profile solely positive for 14% of tested colon cancer sera. Through these five antigens, KY-CC-17/β-actin has cytoskeleton function; KY-CC-14/ACTR1A and KY-CC-19/TSGA2 participate in chromosome segregation; KY-CC-12/FKBP4 regulates steroid receptor function and KY-CC-15/PLRG1 is a component of spliceosome complex. For the last four antigens tested were found aberrant mRNA expression in some cases of colon tumor. Conclusion: The exploration of identified antigens may define suitable targets for immunotherapy or diagnostic of colon cancer. Key Words: colon cancer, SEREX-analysis, allogeneic immunoscreening. |
| format |
Article |
| author |
Garifulin, O.M. Kykot, V.O. Gridina, N.Y. Kiyamova, R.G. Gout, I.T. Filonenko, V.V. |
| author_facet |
Garifulin, O.M. Kykot, V.O. Gridina, N.Y. Kiyamova, R.G. Gout, I.T. Filonenko, V.V. |
| author_sort |
Garifulin, O.M. |
| title |
Application of serex-analysis for identification of human colon cancer antigens |
| title_short |
Application of serex-analysis for identification of human colon cancer antigens |
| title_full |
Application of serex-analysis for identification of human colon cancer antigens |
| title_fullStr |
Application of serex-analysis for identification of human colon cancer antigens |
| title_full_unstemmed |
Application of serex-analysis for identification of human colon cancer antigens |
| title_sort |
application of serex-analysis for identification of human colon cancer antigens |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2015 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145484 |
| citation_txt |
Application of serex-analysis for identification of human colon cancer antigens / O.M. Garifulin, V.O. Kykot, N.Y. Gridina, R.G. Kiyamova, I.T. Gout, V.V. Filonenko // Experimental Oncology. — 2015. — Т. 37, № 3. — С. 173-180. — Бібліогр.: 46 назв. — англ. |
| series |
Experimental Oncology |
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2023-05-20T17:22:16Z |
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2023-05-20T17:22:16Z |
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