Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats
Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determ...
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| Дата: | 2018 |
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| Автори: | , , , , |
| Формат: | Стаття |
| Мова: | English |
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Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України
2018
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| Назва видання: | Experimental Oncology |
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| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/145710 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. |
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irk-123456789-1457102019-01-27T01:23:43Z Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. Original contributions Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity. 2018 Article Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. 1812-9269 http://dspace.nbuv.gov.ua/handle/123456789/145710 en Experimental Oncology Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Original contributions Original contributions |
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Original contributions Original contributions Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats Experimental Oncology |
| description |
Aim: To investigate the influence of exogenous lactoferrin (LF) on tumor growth, energy and lipid metabolism of Walker-256 carcinosarcoma and to assess genotoxic effects of LF. Materials and Methods: The study was performed on Walker-256 tumor-bearing rats. Total lipids and phospholipids were determined by thin-layer chromatography. Comet assay was used to investigate the genotoxic effects of LF. Results: Daily i.p. administrations of exogenous LF at concentrations of 1 mg/kg and 10 mg/kg starting from the 4th day after tumor transplantation suppressed growth of Walker-256 carcinosarcoma by almost 44%. After treatment with recombinant LF in both doses, the phospholipid composition of Walker-256 carcinosarcoma cells was changed (3-fold increase of phosphatidylethanolamine, 3.4-fold increase of phosphatidylcholine, and 1.8-fold increase of sphingomyelin, while the cardiolipin content decreased by 67%. Exogenous LF was not genotoxic for bone marrow cells (as assessed by the ratio of PCE/NCE, number of micronuclei) and peripheral blood lymphocytes (percentage of DNA in the tail of a comet) in Walker-256 carcinosarcoma-bearing rats. Conclusion: Exogenous LF caused the inhibition of Walker-256 carcinosarcoma growth and a decrease in the microviscosity of plasma cell membranes, and exerted no genotoxicity toward bone marrow cells and peripheral blood of experimental animals. Key Words: lactoferrin, breast cancer, Walker-256 carcinosarcoma, phospholipid content, genotoxicity. |
| format |
Article |
| author |
Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. |
| author_facet |
Chekhun, V.F. Storchai, D.M. Todor, I.N. Borikun, T.V. Lukianova, N.Yu. |
| author_sort |
Chekhun, V.F. |
| title |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_short |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_full |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_fullStr |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_full_unstemmed |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats |
| title_sort |
antitumor and genotoxic effects of lactoferrin in walker-256 tumor-bearing rats |
| publisher |
Інститут експериментальної патології, онкології і радіобіології ім. Р.Є. Кавецького НАН України |
| publishDate |
2018 |
| topic_facet |
Original contributions |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/145710 |
| citation_txt |
Antitumor and genotoxic effects of lactoferrin in Walker-256 tumor-bearing rats / V.F. Chekhun, D.M. Storchai, I.N. Todor, T.V. Borikun, N.Yu. Lukianova // Experimental Oncology. — 2018 — Т. 40, № 3. — С. 200–204. — Бібліогр.: 28 назв. — англ. |
| series |
Experimental Oncology |
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| first_indexed |
2023-05-20T17:22:52Z |
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2023-05-20T17:22:52Z |
| _version_ |
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