Time Profile of nNOS Expression in the Spinal Dorsal Horn After L₅ Spinal Root Transection in Rats
Using immunohistochemical analysis, we investigated the time profile of neuronal nitric oxide synthase (nNOS) expression in the lumbar spinal cord up to day 28 after transection of the L₅ spinal root. On day 14 after injury, we also evaluated the effect of intrathecal application of 7-nitroinda...
Збережено в:
Видавець: | Інститут фізіології ім. О.О. Богомольця НАН України |
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Дата: | 2015 |
Автори: | , , , , , , |
Формат: | Стаття |
Мова: | English |
Опубліковано: |
Інститут фізіології ім. О.О. Богомольця НАН України
2015
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Назва видання: | Нейрофизиология |
Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/148209 |
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Цитувати: | Time Profile of nNOS Expression in the Spinal Dorsal Horn After L₅ Spinal Root Transection in Rats / Z. Bahari, H. Manaheji, L. Dargahi, S. Daniali, M. Norozian, G.H. Meftahi, M. Sadeghi // Нейрофизиология. — 2015. — Т. 47, № 4. — С. 341-348. — Бібліогр.: 22 назв. — англ. |
Репозиторії
Digital Library of Periodicals of National Academy of Sciences of UkraineРезюме: | Using immunohistochemical analysis, we investigated the time profile of neuronal nitric
oxide synthase (nNOS) expression in the lumbar spinal cord up to day 28 after transection
of the L₅
spinal root. On day 14 after injury, we also evaluated the effect of intrathecal
application of 7-nitroindazole (7-NI), a selective nNOS inhibitor (8.15 µg in 5 µl), on thermal
hyperalgesia. Our results indicated that nerve transection increased the intensity of nNOSimmunoreactivity in superficial and deep laminae of the dorsal horn within a late stage (days
7 to 28) of the neuropathy model used. Furthermore, 7-NI attenuated nerve injury-evoked
thermal hypersensitivity on day 14 but did not reduce it between days 2 and 5 after transection.
These data suggest that nNOS overexpression is more involved in the development than in the
initiation of thermal hyperalgesia in L₅
-transected rats. |
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