Heterozygous deletions are main cause of expression alterations of PPM1M and PRICLE2 genes in human clear cell renal cell carcinomas

• Дата: 2015
• Автори: Rudenko, E.E., Lapska, Y.V., Gerashchenko, G.V., Stakhovsky, E.O., Vikarchuk, M.V., Kashuba, V.I.
• Формат: Стаття
• Мова: English
• Опубліковано: 2015
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152430
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Heterozygous deletions are main cause of expression alterations of PPM1M and PRICLE2 genes in human clear cell renal cell carcinomas / E.E. Rudenko, Y.V. Lapska, G.V. Gerashchenko, E.O. Stakhovsky, M.V. Vikarchuk, V.I. Kashuba // Biopolymers and Cell. — 2015. — Т. 31, № 1. — С. 29-33 . — Бібліогр.: 21 назв. — англ.

Aim. To discover the mechanisms of the PPM1M and PRICKLE2 genes expression alterations in clear cell renal cell carcinomas. Methods. Study of the copy number was performed, using the quantitative PCR (Q-PCR). Results. Deletions of PPM1M were found in 55 % of cases, amplifications – in 17 % and in 28 % of samples there were no changes of the copy number. We found the deletions of PRICKLE2 in 50 % of samples, no changes of the copy number in 39 %, and amplifications in 11 % of cases. Conclusions. By the analysis of the gene copy number we have shown that homo- and heterozygous deletions are the main reason of changes in expression of the PPM1M and PRICKLE2 genes.