Expression of isgylation related genes in regenerating rat liver

• Дата: 2015
• Автори: Kuklin, A.V., Poliezhaieva, T.A., Zhyryakova, I.O., Ogryzko, V.V., Obolenskaya, M.Yu.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2015
• Назва видання: Вiopolymers and Cell
• Теми: Genomics, Transcriptomics and Proteomics
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152596
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Expression of isgylation related genes in regenerating rat liver / A.V. Kuklin, T.A. Poliezhaieva, I.O. Zhyryakova, V.V. Ogryzko, M.Yu. Obolenskaya // Вiopolymers and Cell. — 2015. — Т. 31, № 5. — С. 351-361. — Бібліогр.: 52 назв. — англ.

Our recent studies have revealed the early up-regulated expression of interferon alpha (IFNα) in the liver, induced by partial hepatectomy. The role of this cytokine of innate immune response in liver regeneration is still controversial. Aim. To analyze expression of canonical interferon-stimulated genes Ube1l, Ube2l6, Trim25, Usp18 and Isg15 during the liver transition from quiescence to proliferation induced by partial hepatectomy, and acute phase response induced by laparotomy. These genes are responsible for posttranslational modification of proteins by ISGylation. The expression of genes encoding TATA binding protein (TBP) and 18S rRNA served as indirect general markers of transcriptional and translational activities. Methods. The abundance of investigated RNAs was assessed in total liver RNA by real time RT–qPCR. Results. Partial hepatecomy induced steady upregulation of the Tbp and 18S rRNA genes expression during 12 hours post-surgery and downregulation or no change in expression of ISGylation-related genes during the first 3 hours followed by slight upregulation at 12 hours. The level of Isg15 transcripts was permanently below that of the control during the prereplicative period. Laparotomy induced a continuous downregulation of Tbp and 18S rRNA expression and early (1–3h) upregulation of ISGylation–related transcripts followed by a sharp drop at 6 hours and slight increase/decrease at 12 hours. The changes in the abundance of Ifnα and ISGylation-related mRNAs were oppositely directed at each stage of the response to partial hepatectomy and laparotomy. Conclusion. We suggest that the expression of ISGylation-related genes does not depend on the expression of Ifnα gene after both surgeries. The indirect indices of transcription and translation as well as the expression of ISGylation-relaled genes are principally different in response to partial hepatectomy and laparotomy and argue for the high specificity of innate immune response.