Heterogeneity of premetastatic niches gene expression in breast cancer cells

• Main Authors: Tashireva, L.A., Denisov, E.V., Savelieva, O.E., Geraschenko, T.S., Zavyalova, M.V., Perelmuter, V.M.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2015
• Series: Вiopolymers and Cell
• Subjects: Genomics, Transcriptomics and Proteomics
• Online Access: http://dspace.nbuv.gov.ua/handle/123456789/152701

Aim. To investigate the expression of the genes TGFB1, TNF, CSF1, CSF2, VEGFA and HIF1A in the patients with invasive breast carcinoma of no special type considering the intratumoral morphological heterogeneity. Methods. The technology of laser capture microdissection PALM was used to isolate five types of morphological tumor structures from three patients with invasive carcinoma of no special type (IC NST), luminal A subtype, T1-2NxMx. The level of expression of the cytokine (TNF), growth factor genes (TGFB1, CSF1, CSF2, VEGFA) and the HIF1A gene was assessed in the samples obtained using real-time PCR, TaqMan-probes and specific oligonucleotides. Results. The study demonstrated the absence of the expression of the growth factor gene CSF2 in tumor cells of IC NST, and the expression of the gene CSF1, independent from the metastasis status and tumor structure type. The prevalence of the expression of the genes VEGFA and TGFB1 was revealed in the alveolar and solid structures along with the rare expression of the gene TNF. Conclusions. The expression of pre-metastatic niche genes in the tumors of patients with IC NST is heterogeneous. The hypoxia-mediated change in the cytokine gene expression may be expected in the alveolar and solid structures, which ultimately results in the formation of microenvironment, facilitating tumor growth and the formation of tumor metastatic potential.