DNA metabarcoding of microbial communities for healthcare

• Дата: 2016
• Автори: Zaets, I.Ye., Podolich, O.V., Reva, O.N., Kozyrovska, N.O.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2016
• Назва видання: Вiopolymers and Cell
• Теми: Reviews
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152749
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: DNA metabarcoding of microbial communities for healthcare / I.Ye. Zaets, O.V. Podolich, O.N. Reva, N.O. Kozyrovska // Вiopolymers and Cell. — 2016. — Т. 32, № 1. — С. 3-8. — Бібліогр.: 34 назв. — англ.

High-throughput sequencing allows obtaining DNA barcodes of multiple species of microorganisms from single environmental samples. Next Generation Sequencing (NGS)-based profiling provides new opportunities to evaluate the human health effect of microbial community members affiliated to probiotics. The DNA metabarcoding may serve to a quality control of microbial communities, comprising complex probiotics and other fermented foods. A detailed inventory of complex communities is a pre-requisite of understanding their functionality as whole entities that makes it possible to design more effective bio-products by precise replacement of one community member by others. The present paper illustrates how the NGS-based DNA metabarcoding aims at the profiling of both wild and hybrid multi-microbial communities with the example of kombucha probiotic beverage fermented by yeast-bacterial partners.