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DNA metabarcoding of microbial communities for healthcare

High-throughput sequencing allows obtaining DNA barcodes of multiple species of microorganisms from single environmental samples. Next Generation Sequencing (NGS)-based profiling provides new opportunities to evaluate the human health effect of microbial community members affiliated to probiotics. T...

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Bibliographic Details
Main Authors: Zaets, I.Ye., Podolich, O.V., Reva, O.N., Kozyrovska, N.O.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2016
Series:Вiopolymers and Cell
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Online Access:http://dspace.nbuv.gov.ua/handle/123456789/152749
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Summary:High-throughput sequencing allows obtaining DNA barcodes of multiple species of microorganisms from single environmental samples. Next Generation Sequencing (NGS)-based profiling provides new opportunities to evaluate the human health effect of microbial community members affiliated to probiotics. The DNA metabarcoding may serve to a quality control of microbial communities, comprising complex probiotics and other fermented foods. A detailed inventory of complex communities is a pre-requisite of understanding their functionality as whole entities that makes it possible to design more effective bio-products by precise replacement of one community member by others. The present paper illustrates how the NGS-based DNA metabarcoding aims at the profiling of both wild and hybrid multi-microbial communities with the example of kombucha probiotic beverage fermented by yeast-bacterial partners.