Extreme evolutionary stability of conserved non-protein coding element of baculovirus genome

• Дата: 2016
• Автори: Makarenko, V.E., Kikhno, I.M., Kashuba, V.I.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2016
• Назва видання: Вiopolymers and Cell
• Теми: Viruses and Cell
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152811
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Extreme evolutionary stability of conserved non-protein coding element of baculovirus genome / V.E. Makarenko, I.M. Kikhno, V.I. Kashuba // Вiopolymers and Cell. — 2016. — Т. 32, № 2. — С. 131-139. — Бібліогр.: 37 назв. — англ.

Aim. To estimate a level of evolutionary stability of the baculovirus conserved non protein-coding element (CNE) playing an essential role in baculovirus pathogenesis. Methods. NCBI-BLAST was applied to identify the orthologous sequences in genomes of 50 alphabaculoviruses. The orthologous sequences were aligned by using ClustalW software. Alistat tool was applied to obtain the pairwise percent identity ( %ID) matrix data. Results. An average pairwise ID value (73 %) calculated for the 1225-member sample was shown to be comparable with those of coding parts of the most conserved baculovirus genes polh and pif2 as well as with those of the polh and p18 promoter regions which are the most conserved representatives of a small group of the evolutionary stable promoter regions of the alphabaculovirus late genes. Conclusion. CNE is one of the most conserved elements of the alphabaculovirus genome.