Development of amperometric biosensor for choline determination

• Дата: 2016
• Автори: Kucherenko, D.Yu., Siediuko, D.V., Knyzhnykova, D.V., Soldatkin, O.O., Soldatkin, A.P.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2016
• Назва видання: Вiopolymers and Cell
• Теми: Molecular and Cell Biotechnologies
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152821
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Development of amperometric biosensor for choline determination / D.Yu. Kucherenko, D.V. Siediuko, D.V. Knyzhnykova, O.O. Soldatkin, A.P. Soldatkin // Вiopolymers and Cell. — 2016. — Т. 32, № 3. — С. 229-234. — Бібліогр.: 9 назв. — англ.

Aim. Development of an amperometric biosensor for measuring choline concentration in water samples. Methods. A bioselective element of the biosensor was created using choline oxidase which was covalently immobilized by glutaraldehyde crosslinking with bovine serum albumin on the surface of an amperometric platinum disk electrode. Results. The conditions of the bioselective element formation (the enzyme and glutaraldehyde concentrations, time of procedure) were optimized. The biosensor developed was characterized by good response reproducibility over hours of continuous operation. The linear range of substrate determination ranged from 10 µM to 1000 µM, a limit of choline detection – 1–3 µM, the biosensor sensitivity was 25–30 nA/mM. An effect of interfering substances was significantly reduced by the application of an additional semipermeable poly-m-phenylenediamine (PPD) membrane. Conclusions. The developed biosensor is well-suited for choline determination in water samples.