Surface-exposed actin binds plasminogen on the membrane of agonist-activated platelets: a flow cytometry study

• Дата: 2017
• Автори: Tykhomyrov, A.A., Zhernossekov, D.D., Grinenko, T.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2017
• Назва видання: Вiopolymers and Cell
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152928
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Surface-exposed actin binds plasminogen on the membrane of agonist-activated platelets: a flow cytometry study / A.A. Tykhomyrov, D.D. Zhernossekov, T.V. Grinenko // Вiopolymers and Cell. — 2017. — Т. 33, № 3. — С. 172-182. — Бібліогр.: 30 назв. — англ.

Aim. To elucidate whether actin exposed on the surface of agonist-activated platelets is responsible for plasminogen binding. Methods. Human washed platelets were obtained by gel-filtration on Sepharose-2B. The levels of exposed actin on the outer surface of plasma membrane of intact and thrombin- or collagen-stimulated platelets were monitored by flow cytometry. The binding of plasminogen fluorescent conjugate (Pg-FITC) with platelets treated with anti-actin antibody or non-immune IgG (control) was analyzed cytometrically. Results. Thrombin or collagen exposure resulted in the dose-dependent actin expression on the surface of stimulated platelets. Pre-treatment of stimulated platelets with specific anti-actin antibodies was shown to significantly prevent the binding of Pg-FITC with the platelet surface. Conclusion. The results of the present study indicate that actin exposed on the platelet surface at the agonist-induced activation is involved in the plasminogen binding.