Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA

• Дата: 2017
• Автор: Polishchuk, L.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2017
• Назва видання: Вiopolymers and Cell
• Теми: Bioinformatics
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/152932
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Similarity and dissimilarity of primary structures of some Streptomyces spp. genomes and the Streptomyces globisporus 1912-2 chromosomal DNA / L.V. Polishchuk // Вiopolymers and Cell. — 2017. — Т. 33, № 3. — С. 206-213. — Бібліогр.: 29 назв. — англ.

Aim. To determine the similarity and dissimilarity of the nucleotide sequences of the S. globisporus 1912-2 chromosome and primary structures of genomes of some Streptomycetes strains. Methods. NCBI tools (BLAST: blastn and bl2seq: megablast) and Internet NCBI databases (Genome, Nucleotide) were used for in silico analysis of the primary structure contigs of S. grlobisporus 1912-2. Results. A few strains with significant identity of their DNA primary structures to the nucleotide sequences of the chromosomal DNA of S. globisporus 1912-2 (identity 88–97 %) and a degree of query cover (55–82 %) were identified. Primary structures of genomes of the strains S. globisporus C-1027 and S. griseus NBRC13350 were chosen as most identical to the nucleotide sequence of the S. globisporus 1912-2 chromosomal DNA. No fragments with a homologous primary structure to seven S. globisporus 1912-2 contigs were found in Streptomycetes spp. from the NCBI databases. Conclusions. S. globisporus 1912-2 strain is a member of the S. griseus clade. We detected a high biosynthetic potential of the strain S. globisporus 1912-2 due to many unique nucleotide sequences.