Comparative analysis of epigenetic markers in plasma and tissue of patients with colorectal cancer

• Main Authors: Kondratov, A.G., Nekrasov, K.A., Lototska, L.V., Panasenko, G.V., Stoliar, L.A., Lapska, Y.V., Kolesnyk, O.O., Shchepotin, I.B., Rynditch, A.V., Kashuba, V.I.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2014
• Series: Вiopolymers and Cell
• Subjects: Biomedicine
• Online Access: http://dspace.nbuv.gov.ua/handle/123456789/153751

Aim. The work is devoted to the development of less invasive tools for the colorectal cancer (CRC) screening. Methods. Q-PCR and methylation-specific PCR techniques were used in the current work. Results. We have shown that the levels of cell-free plasma DNA are higher in the CRC patients compared with the healthy donors (p < 0.01). Hypermethylation of APC, FHIT, LRRC3B and HIC1 genes was studied in the tumor and plasma samples of CRC patients. Two-stage verification for CRC screening was proposed. Conclusions. We proposed and tested a novel approach for CRC screening based on the determination of cell-free DNA and methylated DNA fragments in the plasma.