Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination
The characteristics of conductometric urease microbiosensor based on thin-film inter-digitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serumalbumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatic...
Збережено в:
| Дата: | 1996 |
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| Автори: | , , , |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
1996
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| Назва видання: | Биополимеры и клетка |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/153794 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | Conductometric urease microbiosensor based on thin-film interdigitated electrodes for urea determination / S.V. Dzyadevich, G.A Zhylyak., A.P. Soldatkin, A.V. El'skaya // Биополимеры и клетка. — 1996. — Т. 12, № 1. — С. 53-57. — Бібліогр.: 9 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | The characteristics of conductometric urease microbiosensor based on thin-film inter-digitated electrodes for urea determination have been studied. Urease has been immobilized by cross-linking with bovine serumalbumin using glutaraldehyde. The resulting conductivity changes are produced by enzymatically catalyzed hydrolysis of urea. This process for both immobilized enzyme and soluble one is described by the classic laws of enzymatic kinetics. The influence of ionic strength, pH and buffer capacity of the samples on the biosensor response has been thoroughly tested. The results have been discussed concerning urea concentration analysis in human blood. |
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