The effect of lipopolysaccharide of Pseudomonas syringae pv. atrofaciens 9417 on mutagenicity in pro- and eukaryotic systems

• Дата: 2010
• Автори: Bogdan, Yu.M., Butsenko, L.M., Pasichnyk, L.A., Gvozdyak, R.I.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2010
• Назва видання: Вiopolymers and Cell
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/153845
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: The effect of lipopolysaccharide of Pseudomonas syringae pv. atrofaciens 9417 on mutagenicity in pro- and eukaryotic systems / Yu.M. Bogdan, L.M. Butsenko, L.A. Pasichnyk, R.I. Gvozdyak, // Вiopolymers and Cell. — 2010. — Т. 26, № 1. — С. 23-28. — Бібліогр.: 18 назв. — англ, укр.

Aim. To study the effect of lipopolysaccharide (LPS) of Pseudomonas syringae pv. atrofaciens on spontaneous and induced mutations in pro- and eukaryotic test-systems. Methods. Mutagenic and antimutagenic properties of LPS were studied in Allium cepa-test and Ames test. Results. LPS does not influence the spontaneous mutations of Salmonella typhimurium and decreases the level of mutations induced by potassium dichromate and N-methyl-N'-nitro-N'-nitrosoguanidine. LPS reduces a mitotic index at concentrations of 10.0 and 5.0 mg/ml and increases the number of chromosomes’ fragments in cells of A. cepa root apical meristem at concentrations of 5.0 and 2.5 mg/ml. Conclusion. Different effect of LPS on mutagenesis in pro- and eukaryotic cells has been established. LPS revealed mutagenic properties in A. cepa-test and antimutagenic properties in Ames test.