Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures

• Дата: 2009
• Автори: Goncharuk, E.I., Borovoy, I.A., Pavlovich, E.V., Malyukin, Yu.V., Grischenko, V.I.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2009
• Назва видання: Вiopolymers and Cell
• Теми: Біоорганічна хімія
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/153846
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Newly synthesized carbocyanine fluorescent probes, their characteristics and behavior in proliferating cultures / E.I. Goncharuk, I.A. Borovoy, E.V. Pavlovich, Yu.V. Malyukin, V.I. Grischenko // Biopolymers and Cell. — 2009. — Т. 25, № 6. — С. 484-490. — Бібліогр.: 17 назв. — англ.

Aim. To study possibile application of C2, C9, C18 and JC-1 carbocyanine fluorescent dyes for cell culture characterization. Methods. Morphological methods, fluorescence-activated cell sorting (FACS) analysis, luminescent microscopy were used. Results. The studied carbocyanine probes were shown to be preserved in dividing cells for at least 4 duplications. It was found that carbocyanine probe JC-1 did not transit from cell to cell under combined culturing of labeled and non-labeled cells. Conclusions. The paper covers the use of carbocyanine fluorescent probes for long-term culturing of cell lines. Probes C9 and JC-1 were optimal for the proliferative culture observation, allowing to trace mitochondrial functional state.