Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress

• Дата: 2010
• Автори: Kopich, V.N., Kretynin, S.V., Kharchenko, O.V., Litvinovskaya, R.P., Chashina, N.M., Khripach, V.A.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2010
• Назва видання: Вiopolymers and Cell
• Теми: Bioorganic Chemistry
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/153894
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Effect of 24-epibrassinolide on lipoxygenase activity in maize seedlings under cold stress / V.N. Kopich, S.V. Kretynin, O.V. Kharchenko, R.P. Litvinovskaya, N.M. Chashina, V.A. Khripach // Вiopolymers and Cell. — 2010. — Т. 26, № 2. — С. 218-224. — Бібліогр.: 34 назв. — англ, укр.

Aim. To investigate 24-epibrassinolide influence on the maize seedlings 9- and 13-lipoxygenases activity (9- and 13-LOX) under normal conditions (25 oC) and cold stress (5 oC). Methods. LOX activity was measured after treatment of seedlings with 0.01 and 1 μM 24-epibrassinolide. The enzymes were extracted from maize seedlings with 0.1 M sodium acetate (pH 4.5) buffer, supplemented with a non-ionic detergent (0.1 % Brij-99) and EDTA (0.1 mM). The 9- and 13-LOX activities were determined spectrophotometrically at 234 nm using linoleic acid as substrate at pH 6.0 and 7.0 in the presence or absence of 0.02 % Lubrol PX. Results. 3-6-fold increase in LOX activity of 24-epibrassinolide-treated seedlings was demonstrated under normal conditions. Cold stress in the presence of 1µM 24-epibrassinolide enhances the activities of 9- and 13- LOX by 4 and 10 times, respectively. Conclusions. The results obtained enlarge our understanding of possible pathways of LOX metabolites involvement in the formation of cell response to brassinosteroids.