Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1

• Дата: 2010
• Автори: Duplij, D.R., Maksymchuk, O.V., Duplij, V.P., Chaschin, N.A.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2010
• Назва видання: Вiopolymers and Cell
• Теми: Short Communications
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/154131
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Comparative analysis of human and mammalias genes of protein ortologs of cytochrome P450 2E1 / D.R. Duplij, O.V. Maksymchuk, V.P. Duplij, N.A. Chaschin // Вiopolymers and Cell. — 2010. — Т. 26, № 4. — С. 322-326. — Бібліогр.: 13 назв. — англ, рос.

Aim. To carry out a comparison analysis of nucleotide sequences for the cytochrome P450 2E1 protein ortologs genes and reestablish the connection of gene evolution with nucleotide content. Methods. In silico: BLAST, ClustalW, MEGA4, PHP. Results. A general phylogeny of CYP2E1 genes is described. The most affinity is found for human translated sequences with Pan troglodytes. The transition C > T is the most often, it occurs in introns by 2.6 times more than in exons. The correlation of keto-amino skew of CYP2E1 genes and evolution age of species is stated. Conclusions. The analysis carried out in the paper allows us to assume that a common ancestor of the CYP2E1 protein isoform lived before the divergence between rodent and Primates orders, i.e. 70 million years ago. The single nucleotide substitution is accumulated in introns during evolution.