Effect of fluorinated N-alkylthioamides on HSV-1 multiplicity

• Дата: 2018
• Автори: Biliavska, L.O., Pankivska, Y.B., Povnitsa, O.Yu., Pikun, N.V., Shermolovich, Y.G., Zagorodnya, S.D
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2018
• Назва видання: Вiopolymers and Cell
• Теми: Viruses and Cell
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/154268
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Effect of fluorinated N-alkylthioamides on HSV-1 multiplicity / L.O. Biliavska, Y.B. Pankivska, O.Yu. Povnitsa, N.V. Pikun, Y.G. Shermolovich, S.D Zagorodnya // Вiopolymers and Cell. — 2018. — Т. 34, № 1. — С. 49-58. — Бібліогр.: 21 назв. — англ.

The number of promising viral targets and classes of compounds with substantial antiherpetic properties considerably increased during the last decade. However, no new effective and low-toxicity clinical drugs against both wild-type viruses and drug-resistant strains have appeared. This situation makes the search for new antiherpetic drugs and their new targets a high priority. Aim. To study the effect of fluorinated N-alkylthioamides on the herpes simplex virus-1 (HSV-1) multiplicity. Methods. The influence of these compounds on the multiplicity and infectivity of HSV-1 was determined by the MTT-assay, virucidal assay, adsorption and penetration assays, PCR and infectious virus yield reduction assay. Results. The 10S-23 and 10S-24 compounds pre-vented the adsorption and penetration of HSV-1 into cells up to 26%. HSV-1 DNA replication was moderately inhibited by compound 10S-24 (to 39 %). It was found that the compounds 10S-23 and 10S-24 in concentration of 100 – 33 μg/ml reduce the titer of virus obtained de novo by 70 – 99% and >99%, respectively. Conclusion. These results suggest that the 10S-24 compound may be used as a therapeutic agent to reduce the penetration, replication and translation of HSV-1.