Chromatin enrichment of histone marks H4Ac and H3K9me3 in TP53 gene domain in breast cells

• Дата: 2014
• Автори: Santos, G.C.Jr, Goes, A.C.S., de Moura Gallo, C.V.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2014
• Назва видання: Вiopolymers and Cell
• Теми: Structure and Function of Biopolymers
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/154304
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Chromatin enrichment of histone marks H4Ac and H3K9me3 in TP53 gene domain in breast cells / G.C.Jr Santos, A.C.S. Goes, C.V. de Moura Gallo // Вiopolymers and Cell. — 2014. — Т. 30, № 3. — С. 197-202. — Бібліогр.: 40 назв. — англ.

In non-cancerous breast cell lines HB2 and MCF10A the TP53 gene is localized inside a relatively small ~ 50 kb loop domain delimited by two S/MARs. Aim. To analyze the chromatin markers H4Ac and H3K9me3 of these two S/MARs and of the TP53 gene P1 promoter in different breast cells lines. Methods. We used chromatin immunoprecipitation (ChIP) to characterize the chromatin status of these S/MARs elements in breast non-cancerous cell lines HB2 and MCF10A and cancerous MCF-7, MDA-MB-231, BT-474 and T47D cell lines, by chromatin enrichment of H4Ac and H3K9me3 epigenetic markers, hallmarks of open and closed chromatin, respectively. Results. We found that these chromatin epigenetic markers are differentially distributed in S/MARs for all analyzed breast cell lines. Conclusions. We found no correlation between S/MARs and chromatin epige- netic status, suggesting that nuclear matrix fixation and chromatin status can be independent. High enrichment of H3K9me3 in the TP53 gene P1 promoter region in MCF-7, could explain lower levels of the TP53 expression, described earlier by our group.