Association of IL8 and IL10 gene allelic variants with ischemic stroke risk and prognosis

• Main Authors: Kucherenko, A.M., Shulzhenko, D.V., Kuznetsova, S.M., Demydov, S.V., Livshits, L.A.
• Format: Article
• Language: English
• Published: Інститут молекулярної біології і генетики НАН України 2014
• Series: Вiopolymers and Cell
• Subjects: Biomedicine
• Online Access: http://dspace.nbuv.gov.ua/handle/123456789/154305

Aim. Evaluating a role of IL8 gene –781 C/T, and IL10 gene –592C/A polymorphisms as genetic markers of ischemic stroke risk. Methods. A case group consisted of 183 patients with ischemic stroke, which were treated in the Brain Vascular Pathology unit of SI «Institute of Gerontology of NAMS of Ukraine». A control group included 88 healthy individuals older than 65 years without any history of ischemic stroke. Genotyping was performed using PCR followed by restriction fragment length polymorphism analysis. Results. Significantly (P < 0,05) higher frequency of IL8 –781T allele carriers in the case group (81,6 %) comparing to the control (70,1%) was revealed. –781T allele carriers have nearly 2-fold increased ischemic stroke development risk (OR = 1.886; 95 % CI: 1.041–3.417). Significantly (P < 0,05) higher frequency of IL10 gene –592C allele carriers was observed in the patients with ischemic stroke (98,2%) comparing to the control (90,7 %). The ischemic stroke development risk in such individuals is 5-fold increased (OR = 5.71; 95 % CI: 1.48–22.11). It was revealed that –592C allele homozygotes with ischemic stroke have more than 2-fold higher improvement (according to the Rankin scale) chances during the first fortnight of treatment (OR = 2,76; 95 % CI: 1,26–6,07). Conclusions. On the basis of the obtained significant differences, IL8 gene –781T and IL10 gene –592C variants may be considered the factors of ischemic stroke hereditary susceptibility. Besides, IL10 gene –592CC genotype is a genetic marker of the patients state positive dynamics during first two weeks of treatment.