2025-02-22T09:35:39-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: Query fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-154557%22&qt=morelikethis&rows=5
2025-02-22T09:35:39-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: => GET http://localhost:8983/solr/biblio/select?fl=%2A&wt=json&json.nl=arrarr&q=id%3A%22irk-123456789-154557%22&qt=morelikethis&rows=5
2025-02-22T09:35:39-05:00 DEBUG: VuFindSearch\Backend\Solr\Connector: <= 200 OK
2025-02-22T09:35:39-05:00 DEBUG: Deserialized SOLR response
Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells

Comparative study on influence of fetal bovine serum and serum of adult rat on cultivation of newborn rat neural cells

Aim. To study the influence of fetal bovine serum and serum of adult rats on behavior of newborn rat isolated neural cells during their cultivation in vitro. Methods. The isolation of neural cells from neonatal rat brain. The determination of the dynamics of cellular monolayer formation. Immunocytoc...

Full description

Saved in:
Bibliographic Details
Main Authors: Sukach, A.N., Shevchenko, M.V., Liashenko, T.D.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2014
Series:Вiopolymers and Cell
Subjects:
Molecular and Cell Biotechnologies
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/154557
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim. To study the influence of fetal bovine serum and serum of adult rats on behavior of newborn rat isolated neural cells during their cultivation in vitro. Methods. The isolation of neural cells from neonatal rat brain. The determination of the dynamics of cellular monolayer formation. Immunocytochemical staining of cells for &beta;-tubulin III, nestin and vimentin. Results. It has been determined that the addition of serum of adult rats to the cultivation medium creates more favorable conditions for survival, attachment and spread of differentiated, and proliferation of the stem/progenitor neural cells of newborn rats during cultivation in vitro compared with the fetal bovine serum. Conclusions. Using the serum of adult rats is preferable for the cultivation of isolated neural cells of newborn rats compared with the fetal bovine serum.

Similar Items