Biological properties of neural crest-derived multipotent stem cells from the bulge region of whisker follicle expanded in new culture conditions

• Дата: 2014
• Автори: Vasyliev, R.G., Rodnichenko, A.E., Zubov, D.A., Rymar, S.Y., Gubar, O.S., Labunets, I.F., Novikova, S.N.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2014
• Назва видання: Вiopolymers and Cell
• Теми: Molecular and Cell Biotechnologies
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/154583
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: Biological properties of neural crest-derived multipotent stem cells from the bulge region of whisker follicle expanded in new culture conditions / R.G. Vasyliev, A.E. Rodnichenko, D.A. Zubov, S.Y. Rymar, O.S. Gubar, I.F. Labunets, S.N. Novikova // Вiopolymers and Cell. — 2014. — Т. 30, № 6. — С. 469-476. — Бібліогр.: 21 назв. — англ.

Aim. The work is aimed at obtaining the culture of neural crest-derived multipotent stem cells (NC-MSCs) in new culture conditions and to investigate their biological properties. Methods. NC-MSCs were grown from the explants of the bulge region of whisker follicle of adult mice. The cell cultures were examined by the following methods: sphere-forming assay, directed multilineage differentiation, CFU assay, immunocytochemistry, flow cytometry, RT-PCR. Results. The obtained NC-MSCs expressed the typical neural crest markers (nestin, Sox10 and Sox2) and were differentiated into adipocytes, osteoblasts and Schwann cells. Under our original growing conditions, the culture of NC-MSCs at the third passage had the following parameters: 66.8 % nestin+, 3.1 % ALDH brigth and 33.3 % clonogenic cells. The NC-MSCs growth rate depended on plating density. EGF and bFGF demonstrated a dose-dependent mitogenic action on NC-MSCs. Conclusions. The proposed approach permits the NC-MSC expansion with the maintenance of their main functional properties. Further optimization of the culture conditions will be based on the use of growth factors and low plating density.