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Ca²⁺ does not affect the binding properties of ITSN1 EH domains

Ca²⁺ does not affect the binding properties of ITSN1 EH domains

ITSN1 is an endocytic scaffold protein implicated in synaptic functioning. Ca²⁺ is known to be important for endo- cytosis in both pre- and post-synaptic terminals. ITSN1 contains two EH (Eps15 homology) domains which possess putative Ca²⁺-binding EF-hand motifs. Aim. To test the effect of Ca²⁺ on t...

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Bibliographic Details
Main Authors: Morderer, D.Ye., Rymarenko, O.V., Skrypkina, I.Ya., Rynditch, A.V.
Format: Article
Language:English
Published: Інститут молекулярної біології і генетики НАН України 2014
Series:Вiopolymers and Cell
Subjects:
Short Communications
Online Access:http://dspace.nbuv.gov.ua/handle/123456789/154596
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Summary:ITSN1 is an endocytic scaffold protein implicated in synaptic functioning. Ca²⁺ is known to be important for endo- cytosis in both pre- and post-synaptic terminals. ITSN1 contains two EH (Eps15 homology) domains which possess putative Ca²⁺-binding EF-hand motifs. Aim. To test the effect of Ca²⁺ on the EH domain binding properties. Methods. His-tag pulldown, Western blotting. Results. Addition of 1.5 mM Ca²⁺ does not affect the binding of the ITSN1 EH domains to the C-terminal fragment of the endocytic protein Epsin 1. Conclusions. The data obtained indicate that Ca²⁺ has no effect on the binding properties of the ITSN1 EH domains.

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