On the interpretation of decay-associated fluorescence spectra in proteins
Calculating decay-associated spectra (DAS) is a common mode of analyzing complex fluorescence decay in proteins. Unfortunately, these spectra are attributed almost exclusively to certain species in the population of macromolecules (retainers), often without sufficient evidence. An alternative explan...
Збережено в:
| Дата: | 2001 |
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| Автор: | |
| Формат: | Стаття |
| Мова: | English |
| Опубліковано: |
Інститут молекулярної біології і генетики НАН України
2001
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| Назва видання: | Біополімери і клітина |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/155228 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | On the interpretation of decay-associated fluorescence spectra in proteins / A.S. Ladokhin // Біополімери і клітина. — 2001. — Т. 17, № 3. — С. 221-224. — Бібліогр.: 14 назв. — англ. |
Репозитарії
Digital Library of Periodicals of National Academy of Sciences of Ukraine| Резюме: | Calculating decay-associated spectra (DAS) is a common mode of analyzing complex fluorescence decay in proteins. Unfortunately, these spectra are attributed almost exclusively to certain species in the population of macromolecules (retainers), often without sufficient evidence. An alternative explanation of decay heterogeneity, based on structural relaxation., was believed to require a negative DAS component. Here we demonstrate that dipolar relaxation of the indole molecule, lacking any rotameric forms, doesn't always result in negative pre-exponential amplitude. We conclude that the shape of DAS alone is not sufficient to differentiate among various causes of heterogeneity of tryptophan fluorescence decay in proteins. |
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