The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts
Highly effective permeabilization/translation system developed recently (Negrutskii et al. (1994) Proc. Natl Acad. Sci. USA 91, 964–968) was used to study the distribution of FITC-labeled a and S submits of translation elongation factor 1 in human fibroblasts. Somewhat granular fluorescent picture o...
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| Дата: | 1997 |
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| Автор: | |
| Формат: | Стаття |
| Мова: | English |
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Інститут молекулярної біології і генетики НАН України
1997
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| Назва видання: | Биополимеры и клетка |
| Теми: | |
| Онлайн доступ: | http://dspace.nbuv.gov.ua/handle/123456789/155640 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Цитувати: | The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts / B.S. Negrutskii // Биополимеры и клетка. — 1997. — Т. 13, № 5. — С. 380-385. — Бібліогр.: 22 назв. — англ. |
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irk-123456789-1556402019-06-18T01:26:21Z The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts Negrutskii, B.S. Клеточная биология Highly effective permeabilization/translation system developed recently (Negrutskii et al. (1994) Proc. Natl Acad. Sci. USA 91, 964–968) was used to study the distribution of FITC-labeled a and S submits of translation elongation factor 1 in human fibroblasts. Somewhat granular fluorescent picture observed for the both proteins distribution during active protein synthesis may be indicative of putative protein synthesis compartments. Exogenous phosphocreatine, an absolutely required agent to support the protein synthesis in permeabilized cells, is shown to influence the EF-1α distribution, while not affecting the EF-1δ. Most of EF-1α under «low energy» condition is found in nucleus. Thus, the possibility of the functional change in EF-la distribution is demonstrated supporting the Idea about transient character of EF-1α involvement into «heavy» EF-1 complex. Фосфокреатин є абсолютно необхідним компонентом суміші для білкового синтезу у безклітинних системах та пермеабілізованих клітинах. Показано, шр під впливом фосфокреатину змінюється локалізація α-субодиниці фактора елонгації трансляції І у пермеабілізованих клітинах вищих еукаріот. У присутності фосфокреатину EF-la має дискретну локалі зацію в цитоплазмі, а за його відсутністю більшість EF-1α локалізовано у ядрі. Розподіл δ-субодиниці EF-1 не залежить від наявності фосфокреатину в складі суміші для пермеабілізації Фосфокреатин абсолютно необходим для обеспечения белкового синтеза в бесклеточных системах и в пермеабилизованных клетках. Показано, что локализация EF-1α в пермеабилизованных клетках высших эукариот зависит от присутствия фосфокреатина. в среде. Распределение EF-1α в цитоплазме в присутствии фосфокреатина носит дискретный характер, тогда как этот белок обнаруживается преимущественно в ядре в отсутствие фосфокреатина. Локализация EF-1δ не зависит от наличия фосфокреатина в смеси для пермеабилизации. 1997 Article The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts / B.S. Negrutskii // Биополимеры и клетка. — 1997. — Т. 13, № 5. — С. 380-385. — Бібліогр.: 22 назв. — англ. 0233-7657 DOI: http://dx.doi.org/10.7124/bc.00049A http://dspace.nbuv.gov.ua/handle/123456789/155640 en Биополимеры и клетка Інститут молекулярної біології і генетики НАН України |
| institution |
Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| collection |
DSpace DC |
| language |
English |
| topic |
Клеточная биология Клеточная биология |
| spellingShingle |
Клеточная биология Клеточная биология Negrutskii, B.S. The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts Биополимеры и клетка |
| description |
Highly effective permeabilization/translation system developed recently (Negrutskii et al. (1994) Proc. Natl Acad. Sci. USA 91, 964–968) was used to study the distribution of FITC-labeled a and S submits of translation elongation factor 1 in human fibroblasts. Somewhat granular fluorescent picture observed for the both proteins distribution during active protein synthesis may be indicative of putative protein synthesis compartments. Exogenous phosphocreatine, an absolutely required agent to support the protein synthesis in permeabilized cells, is shown to influence the EF-1α distribution, while not affecting the EF-1δ. Most of EF-1α under «low energy» condition is found in nucleus. Thus, the possibility of the functional change in EF-la distribution is demonstrated supporting the Idea about transient character of EF-1α involvement into «heavy» EF-1 complex. |
| format |
Article |
| author |
Negrutskii, B.S. |
| author_facet |
Negrutskii, B.S. |
| author_sort |
Negrutskii, B.S. |
| title |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| title_short |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| title_full |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| title_fullStr |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| title_full_unstemmed |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| title_sort |
effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| publishDate |
1997 |
| topic_facet |
Клеточная биология |
| url |
http://dspace.nbuv.gov.ua/handle/123456789/155640 |
| citation_txt |
The effect of phosphocreatine on the distribution of different subunits of translation elongation factor 1 in gently permeabilized human fibroblasts / B.S. Negrutskii // Биополимеры и клетка. — 1997. — Т. 13, № 5. — С. 380-385. — Бібліогр.: 22 назв. — англ. |
| series |
Биополимеры и клетка |
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2023-05-20T17:47:33Z |
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2023-05-20T17:47:33Z |
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