FSHD myoblasts fail to downregulate intermediate filament protein vimentin during myogenic differentiation

• Дата: 2011
• Автори: Dmitriev, P.V., Barat, A.L., Cochet, E., Ogryzko, V.V., Laoudj-Chenivesse, D., Vassetzky, Y.S., Lipinski, M.
• Формат: Стаття
• Мова: English
• Опубліковано: Інститут молекулярної біології і генетики НАН України 2011
• Назва видання: Вiopolymers and Cell
• Онлайн доступ: http://dspace.nbuv.gov.ua/handle/123456789/155682
• Назва журналу: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Цитувати: FSHD myoblasts fail to downregulate intermediate filament protein vimentin during myogenic differentiation / P.V. Dmitriev, A.L. Barat, E. Cochet, V.V. Ogryzko, D. Laoudj-Chenivesse, M. Lipinski, Y.S. Vassetzky // Вiopolymers and Cell. — 2011. — Т. 27, № 5. — С. 359-363. — Бібліогр.: 25 назв. — англ.

Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant hereditary neuromuscular disorder. The clinical features of FSHD include weakness of the facial and shoulder girdle muscles followed by wasting of skeletal muscles of the pelvic girdle and lower extremities. Although FSHD myoblasts grown in vitro can be induced to differentiate into myotubes by serum starvation, the resulting FSHD myotubes have been shown previously to be morphologically abnormal. Aim. In order to find the cause of morphological anomalies of FSHD myotubes we compared in vitro myogenic differentiation of normal and FSHD myoblasts at the protein level. Methods. We induced myogenic differentiation of normal and FSHD myoblasts by serum starvation. We then compared protein extracts from proliferating myoblasts and differentiated myotubes using SDS-PAGE followed by mass spectrometry identification of differentially expressed proteins. Results. We demonstrated that the expression of vimentin was elevated at the protein and mRNA levels in FSHD myotubes as compared to normal myotubes. Conclusions. We demonstrate for the first time that in contrast to normal myoblasts, FSHD myoblasts fail to downregulate vimentin after induction of in vitro myogenic differentiation. We suggest that vimentin could be an easily detectable marker of FSHD myotubes. Keywords: FSHD, vimentin, myogenic differentiation, proteomics.